Barth Peter J, Schenck zu Schweinsberg Titus, Ramaswamy Annette, Moll Roland
Institute of Pathology, Philipps-University Marburg, Baldingerstrasse, 35033 Marburg, Germany.
Virchows Arch. 2004 Mar;444(3):231-4. doi: 10.1007/s00428-003-0965-1. Epub 2004 Jan 31.
We investigated tumor-free mucosa and squamous cell carcinomas of the oral cavity, the pharynx, and larynx with respect to the presence of stromal CD34+ fibrocytes and alpha-smooth muscle antigen (SMA)-positive myofibroblasts. Additionally, stromal expression of CD117 was analyzed. A total of 39 squamous cell carcinomas were assessed immunohistochemically. In all cases investigated, CD34+ fibrocytes were found in the tumor-free stroma, whereas alpha-SMA-positive myofibroblasts were lacking. Areas of lymphocytic infiltration disclosed a focal reduction of CD34+ fibrocytes. CD117 expression was absent from the tumor-free stroma. Of 39 squamous cell carcinomas, 33 were free of stromal CD34+ fibrocytes, and, in 31 carcinomas, stromal alpha-SMA-positive myofibroblasts occurred at least focally. CD117-positive stromal spindle cells were found in 25 carcinomas. Compared with tumor-free mucosa, the number of tissue mast cells was significantly increased in carcinomas. We conclude that stromal remodeling induced by invasive carcinomas is characterized by a loss of CD34+ fibrocytes and subsequent gain of alpha-SMA-positive myofibroblasts. The diagnostic impact of this finding is, however, limited by the fact that chronic inflammation may also be accompanied by a focal loss of CD34+ fibrocytes.
我们研究了口腔、咽和喉的无肿瘤黏膜及鳞状细胞癌，以了解基质中CD34 + 纤维细胞和α - 平滑肌肌动蛋白（SMA）阳性肌成纤维细胞的存在情况。此外，还分析了CD117的基质表达。总共对39例鳞状细胞癌进行了免疫组织化学评估。在所有研究病例中，无肿瘤基质中均发现了CD34 + 纤维细胞，而缺乏α - SMA阳性肌成纤维细胞。淋巴细胞浸润区域显示CD34 + 纤维细胞局部减少。无肿瘤基质中不存在CD117表达。在39例鳞状细胞癌中，33例无基质CD34 + 纤维细胞，31例癌中至少局部出现基质α - SMA阳性肌成纤维细胞。25例癌中发现了CD117阳性基质梭形细胞。与无肿瘤黏膜相比，癌组织中肥大细胞数量显著增加。我们得出结论，浸润性癌诱导的基质重塑特征是CD34 + 纤维细胞减少，随后α - SMA阳性肌成纤维细胞增加。然而，这一发现的诊断意义受到慢性炎症也可能伴有CD34 + 纤维细胞局部减少这一事实的限制。
