植入前小鼠胚胎培养对行为的长期影响。
Long-term effects of culture of preimplantation mouse embryos on behavior.
作者信息
Ecker David J, Stein Paula, Xu Zhe, Williams Carmen J, Kopf Gregory S, Bilker Warren B, Abel Ted, Schultz Richard M
机构信息
Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104, USA.
出版信息
Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1595-600. doi: 10.1073/pnas.0306846101. Epub 2004 Jan 27.
Abstract
Many procedures used in assisted reproductive technologies (ART) to treat human infertility entail culture of preimplantation embryos. Moreover, there is an increasing trend to culture embryos for longer periods of time before uterine transfer to identify the "best" embryos for transfer and to minimize multiple pregnancies. Embryo culture, however, can perturb embryo metabolism and gene expression, and the long-term consequences of culture are unknown. We have explored the behavioral consequences of embryo culture by using a 129S6/SvEvTac/C57BL/6J F(1) mouse model and find that adults derived from cultured embryos exhibit specific behavioral alterations in the elevated zero maze and Morris water maze tasks.
摘要
许多用于辅助生殖技术（ART）治疗人类不孕症的程序都需要对植入前胚胎进行培养。此外，在子宫移植前延长胚胎培养时间以确定“最佳”移植胚胎并尽量减少多胎妊娠的趋势日益增加。然而，胚胎培养会扰乱胚胎代谢和基因表达，其长期后果尚不清楚。我们通过使用129S6/SvEvTac/C57BL/6J F(1)小鼠模型探索了胚胎培养的行为后果，发现源自培养胚胎的成年小鼠在高架零迷宫和莫里斯水迷宫任务中表现出特定的行为改变。
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