Crystallization of YloQ, a GTPase of unknown function essential for Bacillus subtilis viability.
作者信息
Cladière Lionel, Blagova Elena, Levdikov Vladimir M, Brannigan James A, Séror Simone J, Wilkinson Anthony J
机构信息
Institut de Génétique et Microbiologie, UMR CNRS 8621, Université Paris-Sud, 91405 Orsay CEDEX, France.
出版信息
Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):329-30. doi: 10.1107/S090744490302626X. Epub 2004 Jan 23.
YloQ is a putative ATP/GTP-binding protein of unknown function identified from the complete sequence of the Bacillus subtilis genome. A gene-knockout programme established that yloQ is one of a set of some 270 indispensable genes for the viability of this organism. Crystals of YloQ have been grown from HEPES-buffered solutions at pH 7.5 containing polyethylene glycol and diffraction data have been collected extending to 2.5 A spacing.
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