Xu Ling, Muench Stephen P, Roujeinikova Anna, Sedelnikova Svetlana E, Rice David W
Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, England.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt 5):435-7. doi: 10.1107/S1744309106011456. Epub 2006 Apr 12.
The Bacillus subtilis YphC gene encodes an essential GTPase thought to be involved in ribosome binding and whose protein product may represent a target for the development of a novel antibacterial agent. Sequence analysis reveals that YphC belongs to the EngA family of GTPases, which uniquely contain two adjacent GTP-binding domains. Crystals of a selenomethionine-incorporated YphC-GDP complex have been grown using the hanging-drop vapour-diffusion method and polyethylene glycol as a precipitating agent. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 62.71, b = 65.05, c = 110.61 angstroms, and have one molecule in the asymmetric unit. Data sets at three different wavelengths were collected on a single crystal to 2.5 angstroms resolution at the Daresbury SRS in order to solve the structure by MAD. Ultimately, analysis of YphC in complex with GDP may allow a greater understanding of the EngA family of essential GTPases.
枯草芽孢杆菌的YphC基因编码一种必需的GTP酶，被认为参与核糖体结合，其蛋白质产物可能是新型抗菌剂开发的靶点。序列分析表明，YphC属于GTP酶的EngA家族，该家族独特地包含两个相邻的GTP结合结构域。已使用悬滴气相扩散法和聚乙二醇作为沉淀剂培养了掺入硒代蛋氨酸的YphC-GDP复合物晶体。这些晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 62.71、b = 65.05、c = 110.61埃，不对称单位中有一个分子。在Daresbury SRS的一块单晶上收集了三个不同波长的数据集，分辨率达到2.5埃，以便通过MAD法解析结构。最终，对与GDP结合的YphC的分析可能有助于更深入地了解必需GTP酶的EngA家族。
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