Ip Denis T M, Chan Siu-Hong, Allen Mark D, Bycroft Mark, Wan David C C, Wong Kam-Bo
Department of Biochemistry, The Chinese University of Hong Kong, Hong Kong SAR, People's Republic of China.
Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):340-1. doi: 10.1107/S0907444903027100. Epub 2004 Jan 23.
A novel orange fluorescent protein, with excitation and emission maxima at 548 and 565 nm, respectively, from the Cnidaria tube anemone Cerianthus sp. has been cloned and overexpressed in Escherichia coli. The orange fluorescent protein has been crystallized by the sitting-drop vapour-diffusion method at 290 K using polyethylene glycol 3350 as a precipitant. A complete set of diffraction data was collected to 2.0 A resolution at 100 K. The crystals belong to the space group R3, with hexagonal unit-cell parameters a = b = 216.947, c = 51.839 A. There are four protein molecules in the asymmetric unit, giving a Matthews coefficient of 2.3 A(3) Da(-1) and a solvent content of 47%.
一种来自刺胞动物管海葵（Cerianthus sp.）的新型橙色荧光蛋白已被克隆并在大肠杆菌中过量表达，其激发和发射最大值分别在548和565 nm。该橙色荧光蛋白通过坐滴气相扩散法，以聚乙二醇3350作为沉淀剂，在290 K下结晶。在100 K时收集了一套完整的衍射数据，分辨率达到2.0 Å。晶体属于空间群R3，六方晶胞参数a = b = 216.947，c = 51.839 Å。不对称单元中有四个蛋白质分子，马修斯系数为2.3 ų Da⁻¹，溶剂含量为47%。
