嗜热栖热菌7号菌株中YjgF/YER057c/UK114家族蛋白ST0811的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7.
作者信息
Miyakawa Takuya, Hatano Ken Ichi, Lee Woo Cheol, Kato Yusuke, Sawano Yoriko, Yumoto Fumiaki, Nagata Koji, Tanokura Masaru
机构信息
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Sep 1;61(Pt 9):828-30. doi: 10.1107/S1744309105023808. Epub 2005 Aug 31.
Abstract
ST0811 from Sulfolobus tokodaii strain 7, a member of the YjgF/YER057c/UK114 protein family, was crystallized by the sitting-drop vapour-diffusion method using PEG 10,000 as precipitant. The crystals diffracted X-rays to beyond 2.0 A resolution using an in-house rotating-anode generator. The crystals belonged to the rhombohedral space group R3, with hexagonal unit-cell parameters a = b = 55.0, c = 223.2 A. The crystals contained two molecules in the asymmetric unit (VM = 2.3 A3 Da(-1)) and had a solvent content of 47%.
摘要
来自嗜热栖热菌7株的ST0811是YjgF/YER057c/UK114蛋白家族的成员之一，采用坐滴气相扩散法，以聚乙二醇10000作为沉淀剂进行结晶。使用内部旋转阳极发生器，晶体的X射线衍射分辨率超过2.0 Å。晶体属于菱面体空间群R3，六方晶胞参数a = b = 55.0，c = 223.2 Å。晶体的不对称单元中含有两个分子（VM = 2.3 Å3 Da-1），溶剂含量为47%。
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