• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.构巢曲霉重组异青霉素N合酶的结晶及初步X射线衍射研究
Protein Sci. 1995 May;4(5):1007-9. doi: 10.1002/pro.5560040521.
2
Crystallization and preliminary X-ray diffraction studies on a recombinant isopenicillin N synthase from Cephalosporium acremonium.顶头孢霉重组异青霉素N合酶的结晶及初步X射线衍射研究
J Mol Biol. 1994 Oct 7;242(5):712-4. doi: 10.1006/jmbi.1994.1622.
3
Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii.刚地弓形虫尿嘧啶磷酸核糖转移酶的表达、纯化、结晶及初步X射线衍射分析
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):347-9. doi: 10.1107/S090744499800821X. Epub 1999 Jan 1.
4
Crystallization and preliminary X-ray analysis of copper amine oxidase from Escherichia coli K-12.大肠杆菌K-12铜胺氧化酶的结晶及初步X射线分析
J Mol Biol. 1994 May 13;238(4):635-7. doi: 10.1006/jmbi.1994.1321.
5
Crystallization and preliminary X-ray diffraction analysis of the human dimeric S-Lac lectin (L-14-II).
J Mol Biol. 1993 Oct 5;233(3):553-5. doi: 10.1006/jmbi.1993.1533.
6
Purification, crystallization, and preliminary X-ray analysis of Bacillus subtilis ferrochelatase.枯草芽孢杆菌铁螯合酶的纯化、结晶及初步X射线分析。
Proteins. 1995 Dec;23(4):607-9. doi: 10.1002/prot.340230419.
7
Crystallization and preliminary X-ray diffraction studies of recombinant human interleukin-5.重组人白细胞介素-5的结晶及初步X射线衍射研究
J Mol Biol. 1993 Feb 20;229(4):1150-2. doi: 10.1006/jmbi.1993.1110.
8
Crystallization and preliminary X-ray analysis of Streptococcus pneumoniae hyaluronate lyase.肺炎链球菌透明质酸裂解酶的结晶及初步X射线分析
J Struct Biol. 1998 Jan;121(1):73-5. doi: 10.1006/jsbi.1998.3963.
9
Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli.来自大肠杆菌的ytfG基因产物的结晶及初步X射线晶体学分析
Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):365-7. doi: 10.1107/S0907444903027781. Epub 2004 Jan 23.
10
Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays.来自玉米的重组细胞分裂素氧化酶的纯化、结晶及初步X射线衍射研究
Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1500-1. doi: 10.1107/S0907444904014787. Epub 2004 Jul 21.

引用本文的文献

1
Anaerobic fixed-target serial crystallography.厌氧固定靶标串行晶体学
IUCrJ. 2020 Aug 21;7(Pt 5):901-912. doi: 10.1107/S2052252520010374. eCollection 2020 Sep 1.
2
Prolyl 4-hydroxylase activity-responsive transcription factors: from hydroxylation to gene expression and neuroprotection.脯氨酰4-羟化酶活性响应转录因子：从羟基化到基因表达及神经保护
Front Biosci. 2008 Jan 1;13:2875-87. doi: 10.2741/2892.
3
Active-site-mediated elimination of hydrogen fluoride from a fluorinated substrate analogue by isopenicillin N synthase.异青霉素N合酶通过活性位点介导从氟化底物类似物中消除氟化氢。
Biochem J. 2004 Sep 1;382(Pt 2):659-66. doi: 10.1042/BJ20040529.

本文引用的文献

1
Crystallization and preliminary X-ray diffraction studies on a recombinant isopenicillin N synthase from Cephalosporium acremonium.顶头孢霉重组异青霉素N合酶的结晶及初步X射线衍射研究
J Mol Biol. 1994 Oct 7;242(5):712-4. doi: 10.1006/jmbi.1994.1622.
2
Time-resolved X-ray diffraction studies of myosin head movements in live frog sartorius muscle during isometric and isotonic contractions.在等长和等张收缩过程中，对活蛙缝匠肌中肌球蛋白头部运动进行时间分辨X射线衍射研究。
J Muscle Res Cell Motil. 1994 Jun;15(3):319-48. doi: 10.1007/BF00123484.
3
Purification of isopenicillin N synthetase.异青霉素N合成酶的纯化
Biochem J. 1984 Sep 15;222(3):789-95. doi: 10.1042/bj2220789.

构巢曲霉重组异青霉素N合酶的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.

作者信息

Roach P L, Schofield C J, Baldwin J E, Clifton I J, Hajdu J

机构信息

Dyson Perrins Laboratory, University of Oxford, United Kingdom.

出版信息

Protein Sci. 1995 May;4(5):1007-9. doi: 10.1002/pro.5560040521.

DOI:10.1002/pro.5560040521
PMID:7663335
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2143123/
Abstract

Recombinant Aspergillus nidulans isopenicillin N synthase was purified from an Escherichia coli expression system. The apoenzyme in the presence of saturating concentrations of MnCl2 could be crystallized by either macro- or microseeding, using the hanging drop vapor diffusion technique with polyethylene glycol 8000 as precipitant. The crystals (0.5-1.0 mm overall dimensions) diffract X-rays to at least 2.0 A resolution at synchrotrons and belong to space group P212121 with unit cell dimensions of a = 59.2 A, b = 127.0 A, and c = 139.6 A. The asymmetric unit contains one dimer, and the solvent content of the crystals is 60%. The crystals are radiation sensitive.

摘要

重组构巢曲霉异青霉素N合成酶是从大肠杆菌表达系统中纯化得到的。在饱和浓度的氯化锰存在下，脱辅基酶可以通过宏观或微观接种，采用悬滴气相扩散技术，以聚乙二醇8000作为沉淀剂进行结晶。这些晶体（总体尺寸为0.5 - 1.0毫米）在同步加速器上能将X射线衍射至至少2.0埃的分辨率，属于空间群P212121，晶胞参数为a = 59.2埃，b = 127.0埃，c = 139.6埃。不对称单元包含一个二聚体，晶体的溶剂含量为60%。这些晶体对辐射敏感。