Thomas Jason M, Ting Richard, Perrin David M
The Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, B.C., Canada V6T-1Z1.
Org Biomol Chem. 2004 Feb 7;2(3):307-12. doi: 10.1039/b310154a. Epub 2004 Jan 14.
Inspired by recent interest in DNAzymes as transition metal ion sensors, a survey of the effects of various transition metals on the intramolecular cleavage rate of an imidazole modified, M(2+)-independent, self-cleaving "9(25)-11" DNA is reported. In particular, 9(25)-11 activity was strongly inhibited by Hg(2+)(K(d)(APP)= 110 +/- 9 nM). It is postulated that the affinity and selectivity of 9(25)-11 for Hg(2+) stems from the fact that this synthetically modified DNAzyme contains imidazoles. This study demonstrates the utility of modified nucleotides in developing DNAzyme sensors for metals ions, especially those for which unmodified nucleic acids might not serve as inherently good ligands.
受近期对作为过渡金属离子传感器的脱氧核酶的关注启发，本文报道了一项关于各种过渡金属对一种咪唑修饰的、不依赖M(2+)的自切割“9(25)-11”DNA分子内切割速率影响的研究。特别地，Hg(2+)强烈抑制9(25)-11的活性（K(d)(APP)= 110 +/- 9 nM）。据推测，9(25)-11对Hg(2+)的亲和力和选择性源于这种经合成修饰的脱氧核酶含有咪唑。这项研究证明了修饰核苷酸在开发用于金属离子的脱氧核酶传感器中的实用性，尤其是对于那些未修饰的核酸可能无法作为固有良好配体的金属离子。
