Mure Pierre-Yves, Galdo Mark, Compagnone Nathalie
Department of Neurological Surgery, Brain and Spinal Injury Center, Laboratory for Spinal Cord Development and Regeneration, University of California at San Francisco, California 94143-0527, USA.
J Neurosurg. 2004 Jan;100(1 Suppl Spine):56-61. doi: 10.3171/spi.2004.100.1.0056.
The authors conducted a study to establish outcomes associated with bladder function in a mouse model of spinal cord injury (SCI) and to assess the sensitivity of these outcomes in determining the efficacy of pharmacological treatments.
A mouse model of moderate contusive SCI was used. Outcome parameters included physiological, behavioral, and morphological measurements. To test the sensitivity of these outcomes, the authors used a dehydroepiandrosterone (DHEA) treatment that they had previously shown to promote neurological recovery effectively after SCI. A behavioral scale was used to identify the day at which autonomic function of the bladder was recovered. The reduction in the daily volume of urine during the period of functional recovery paralleled this scale. They then determined the day postinjury at which the functional differences between the vehicle- and DHEA-treated mice exhibited the maximal amplitude. Changes were measured in the composition of the extracellular matrix relative to collagen expression in the layer muscularis of the detrusor at this time point. They found that SCI increases the ratio of collagen type III to collagen type I in the detrusor. Moreover, in the DHEA-treated group, this ratio was similar to that demonstrated in sham-operated mice, establishing the sensitivity of this outcome to assess therapeutic benefits to the bladder function. They next examined the relationship between measurements of neurological recovery and controlled voiding by using cluster analysis.
The authors found that early recovery of controlled voiding is predictive of motor recovery.
作者进行了一项研究，以确定脊髓损伤（SCI）小鼠模型中与膀胱功能相关的结果，并评估这些结果在确定药物治疗疗效方面的敏感性。
使用中度挫伤性SCI小鼠模型。结果参数包括生理、行为和形态学测量。为了测试这些结果的敏感性，作者使用了一种脱氢表雄酮（DHEA）治疗方法，他们之前已证明该方法能在SCI后有效促进神经恢复。使用行为量表来确定膀胱自主功能恢复的日期。功能恢复期间每日尿量的减少与该量表平行。然后，他们确定了损伤后第几天，此时接受载体和DHEA治疗的小鼠之间的功能差异表现出最大幅度。在这个时间点，测量相对于逼尿肌肌层中胶原蛋白表达的细胞外基质组成的变化。他们发现SCI会增加逼尿肌中III型胶原蛋白与I型胶原蛋白的比例。此外，在DHEA治疗组中，该比例与假手术小鼠中的比例相似，这确立了该结果在评估对膀胱功能的治疗益处方面的敏感性。接下来，他们使用聚类分析研究了神经恢复测量与可控排尿之间的关系。
作者发现可控排尿的早期恢复可预测运动恢复。
