Zeng F-S, Zhang L, Cui B-J, Huang L-G, Zhang Q, Sun M, Liu B-L, Meng F, Li Q, Wang D-Q, Sun Q-S
Department of Rehabilitation Medicine, Second Hospital of Shandong University, Jinan, China.
Department of Rehabilitation Medicine, People'S Hospital of Rizhao, Rizhao, China.
Spinal Cord. 2017 Sep;55(9):834-839. doi: 10.1038/sc.2017.37. Epub 2017 Apr 11.
Experimental study.
To investigate the expression of autophagy in different stages of the neurogenic bladder after spinal cord injury (SCI) in rats.
Second Hospital of Shandong University, Jinan, China.
A total of 36 Wistar rats were divided into the SCI and control groups. In total, six animals were killed and sampled from each group at 1, 4 and 14 days after surgery of T10-T11 level. BBB scale, residual urine volume and urinary bladder function score were estimated at each time point. The expression of microtubule-associated protein 1 light chain 3 (LC3) and P62 was detected using western blot analysis, immunofluorescence staining or real-time PCR (RT-PCR).
The locomotor functions of the hindlimbs and the bladder function of the SCI group rats were lost after surgery, but gradually recovered from 1 day. Western blot showed that the LC3-II/actin was higher in the SCI than in the control group. Immunofluorescence staining revealed that LC3 and P62 were expressed in bladder smooth muscle cell. RT-PCR showed a remarkably increased LC3 mRNA expression at 1, 4 and 14 days in the SCI than in the control group. The P62 mRNA level of the SCI bladder tissues did not differ from that of the control group at 1 day but decreased at 4 and 14 days after surgery.
Autophagy is activated during the recovery of the bladder after SCI and sustained. Autophagy may play an important role in bladder neurogenesis and may represent one of the mechanisms of bladder self-repair.
实验性研究。
探讨大鼠脊髓损伤（SCI）后神经源性膀胱不同阶段自噬的表达情况。
中国济南山东大学第二医院。
将36只Wistar大鼠分为SCI组和对照组。在T10 - T11节段手术后1天、4天和14天，每组分别处死6只动物并取样。在每个时间点评估BBB评分、残余尿量和膀胱功能评分。采用蛋白质免疫印迹分析、免疫荧光染色或实时聚合酶链反应（RT-PCR）检测微管相关蛋白1轻链3（LC3）和P62的表达。
SCI组大鼠术后后肢运动功能和膀胱功能丧失，但从术后1天开始逐渐恢复。蛋白质免疫印迹显示，SCI组中LC3-II/肌动蛋白高于对照组。免疫荧光染色显示，LC3和P62在膀胱平滑肌细胞中表达。RT-PCR显示，SCI组在术后1天、4天和14天LC3 mRNA表达明显高于对照组。SCI膀胱组织的P62 mRNA水平在术后1天与对照组无差异，但在术后4天和14天降低。
自噬在SCI后膀胱恢复过程中被激活并持续存在。自噬可能在膀胱神经再生中起重要作用，可能是膀胱自我修复的机制之一。
