Silva Alexandre R, Morato Ronaldo G, Silva Lúcia D M
Laboratory of Carnivore Reproduction, PPGCV-UECE, Paranjana Ave. 1700, Itaperi, Fortaleza, Ceará 60740-000, Brazil.
Anim Reprod Sci. 2004 Mar;81(1-2):159-75. doi: 10.1016/j.anireprosci.2003.10.001.
Species are becoming extinct at a rate 100 times the natural background rates. Considering all mammalian orders, 24% of all Carnivora species are threatened. The goal of carnivore conservation is to reverse the decline in populations and to secure remaining populations in ways that will assure enduring public support. In this context, biotechnology is a tool with tremendous potential for assisting the conservation of endangered canid and felid species. As the first step for biotechnology development is the gamete obtainment, this review will discuss the potential of gamete recovery from non-domestic canids and felids, based on learning how to apply these procedures in the domestic carnivores. Thus, electroejaculation and obtaining both epidydimal spermatozoon and spermatogonial germ cells are indicated as techniques for male gametes recovery. In the female gametes retrieval, different methods for oocyte recovery from both antral and preantral follicles, and the possibility for ovarian tissue transplantation are discussed. Furthermore, the study discusses the responsibilities involved in the use of assisted reproduction in endangered species conservation.
物种灭绝的速度是自然背景速度的100倍。在所有哺乳动物目中，24%的食肉动物物种受到威胁。食肉动物保护的目标是扭转种群数量的下降趋势，并以确保公众持续支持的方式保护剩余种群。在这种背景下，生物技术是一种在协助保护濒危犬科和猫科动物方面具有巨大潜力的工具。由于生物技术发展的第一步是获取配子，本综述将基于学习如何在 domestic carnivores 中应用这些程序，讨论从非 domestic canids 和 felids 中回收配子的潜力。因此，电刺激采精以及获取附睾精子和精原生殖细胞被视为回收雄性配子的技术。在回收雌性配子方面，讨论了从窦状卵泡和窦前卵泡中回收卵母细胞的不同方法以及卵巢组织移植的可能性。此外，该研究还讨论了在濒危物种保护中使用辅助生殖所涉及的责任。
