Luvoni Gaia Cecilia
Department of Veterinary Clinical Sciences, Obstetrics and Gynaecology, University of Milan, Via Celoria 10, 20133 Milan, Italy.
Theriogenology. 2006 Jul 1;66(1):101-11. doi: 10.1016/j.theriogenology.2006.03.012. Epub 2006 Apr 18.
Cryopreservation of gametes is an important tool for the improvement of assisted reproductive technologies. In-depth studies of spermatozoon and oocyte characteristics are required in order to define efficient protocols for the maintenance of viability, including fertilizing and developmental ability, of gametes after thawing. In the domestic cat, semen cryopreservation techniques still produce variable results, the cryopreservation of oocytes is at an experimental level and there have been only a few attempts at cryopreserving gonadal tissue. However, each procedure has generated promising results and has important implications, both for improving reproductive performance of valuable breeds of domestic cats and for conservation of biodiversity in endangered felids by reclamation of valuable male and female germplasm.
配子的冷冻保存是改进辅助生殖技术的一项重要手段。为了确定有效的方案来维持解冻后配子的活力,包括受精和发育能力,需要对精子和卵母细胞的特性进行深入研究。在家猫中,精液冷冻保存技术的结果仍然存在差异,卵母细胞的冷冻保存尚处于实验阶段,而冷冻保存性腺组织的尝试也仅有少数几次。然而,每一项操作都已产生了令人鼓舞的结果,并且对于提高家猫优良品种的繁殖性能以及通过回收珍贵的雄性和雌性种质资源来保护濒危猫科动物的生物多样性都具有重要意义。