Potter S M, Astbury K, Morrison J J
Department of Obstetrics and Gynaecology, National University of Ireland Galway, Clinical Science Institute, University College Hospital, Galway, Ireland.
Am J Obstet Gynecol. 2004 Jan;190(1):246-51. doi: 10.1016/s0002-9378(03)00853-6.
The purpose of this study was to investigate the direct effects of thyrotropin-releasing hormone on isolated human myometrium that was obtained during pregnancy and on human umbilical vasculature in vitro.
Isolated human myometrial strips were dissected from biopsy specimens that were obtained at elective cesarean delivery and suspended for isometric recording under physiologic conditions. The effects of cumulative additions of thyrotropin-releasing hormone (10(-9)-10(-4) mol/L) on oxytocin-induced myometrial contractility were evaluated. The effects of thyrotropin-releasing hormone (10(-9)-10(-4) mol/L) on umbilical vessel (artery and vein) resistance in vitro were investigated with the use of isolated ring preparations.
Thyrotropin-releasing hormone exerted a significant concentration-dependent relaxant effect on pregnant human myometrial tissue, which ranged from 3.54% (10(-9) mol/L, P=.935) to a net cumulative total of 21.06% (10(-4) mol/L, P<.001). Thyrotropin-releasing hormone also exerted a concentration-dependent relaxant effect on human umbilical vasculature that ranged from 12.51% (10(-9) mol/L, P=.994) to a net cumulative total of 23.27%+/-4.87% (SEM, 10(-4) mol/L, P<.01) in umbilical artery. For umbilical vein, the relaxant effect ranged from 1.80% (10(-9) mol/L, P=.998) to a net cumulative total of 14.64% (10(-4) mol/L, P<.009).
Thyrotropin-releasing hormone exerts a significant relaxant effect in human myometrium and in human umbilical vasculature and highlights a potential physiologic role for this neuropeptide in these tissues. These findings have clinical implications for the therapeutic use of thyrotropin-releasing hormone antenatally.
本研究旨在探讨促甲状腺激素释放激素对孕期获取的离体人子宫肌层以及体外人脐血管的直接作用。
从择期剖宫产获取的活检标本中分离出离体人子宫肌条，在生理条件下进行等长记录。评估促甲状腺激素释放激素（10⁻⁹ - 10⁻⁴ mol/L）累积添加对催产素诱导的子宫肌层收缩性的影响。使用离体环行标本研究促甲状腺激素释放激素（10⁻⁹ - 10⁻⁴ mol/L）对体外脐血管（动脉和静脉）阻力的影响。
促甲状腺激素释放激素对孕人子宫肌层组织具有显著的浓度依赖性舒张作用，范围从3.54%（10⁻⁹ mol/L，P = 0.935）到净累积总量21.06%（10⁻⁴ mol/L，P < 0.001）。促甲状腺激素释放激素对人脐血管也具有浓度依赖性舒张作用，在脐动脉中范围从12.51%（10⁻⁹ mol/L，P = 0.994）到净累积总量23.27% ± 4.87%（标准误，10⁻⁴ mol/L，P < 0.01）。对于脐静脉，舒张作用范围从1.80%（10⁻⁹ mol/L，P = 0.998）到净累积总量14.64%（10⁻⁴ mol/L，P < 0.009）。
促甲状腺激素释放激素在人子宫肌层和人脐血管中发挥显著的舒张作用，突出了这种神经肽在这些组织中的潜在生理作用。这些发现对产前促甲状腺激素释放激素的治疗应用具有临床意义。
