Moynihan Audrey T, Hehir Mark P, Glavey Siobhan V, Smith Terry J, Morrison John J
Department of Obstetrics and Gynaecology, Clinical Science Institute, University College Hospital Galway, National University of Ireland, Galway, Ireland.
Am J Obstet Gynecol. 2006 Aug;195(2):504-9. doi: 10.1016/j.ajog.2006.01.106. Epub 2006 May 2.
The purpose of this study was to investigate the effects of leptin on human uterine contractility in vitro.
Biopsies of human myometrium were obtained at elective cesarean section (n = 18). Dissected myometrial strips suspended under isometric conditions, undergoing spontaneous and oxytocin-induced contractions, were exposed to cumulative additions of leptin in the concentration range of 1 nmol/L to 1 micromol/L. Control strips were run simultaneously. Integrals of contractile activity were measured using the PowerLab hardware unit and Chart v3.6 software.
Leptin exerted a potent and cumulative inhibitory effect on spontaneous and oxytocin-induced contractions compared to control strips. The mean maximal inhibition values were as follows: 46.794 +/- 5.133% (n = 6; P < .001) for spontaneous contractions and 42.323 +/- 3.692% (n = 6; P < .001) for oxytocin-induced contractions. There was an apparent reduction in both frequency and amplitude of contractions.
This physiologic inhibitory effect of leptin on uterine contractility may play a role in the dysfunctional labor process associated with maternal obesity, and the resultant high cesarean section rates.
本研究旨在探讨瘦素对人子宫体外收缩力的影响。
在择期剖宫产时获取人子宫肌层活检组织（n = 18）。将分离的子宫肌条在等长条件下悬挂，使其经历自发收缩和催产素诱导的收缩，并暴露于浓度范围为1 nmol/L至1 μmol/L的瘦素累积添加物中。同时对对照肌条进行实验。使用PowerLab硬件单元和Chart v3.6软件测量收缩活动积分。
与对照肌条相比，瘦素对自发收缩和催产素诱导的收缩均产生了强大且累积的抑制作用。平均最大抑制值如下：自发收缩为46.794 +/- 5.133%（n = 6；P <.001），催产素诱导的收缩为42.323 +/- 3.692%（n = 6；P <.001）。收缩的频率和幅度均明显降低。
瘦素对子宫收缩力的这种生理抑制作用可能在与母体肥胖相关的分娩功能障碍过程以及由此导致的高剖宫产率中起作用。
