Sandi C, Cambronero J C, Borrell J, Guaza C
Cajal Institute, CSIC, Madrid, Spain.
Neuroendocrinology. 1992 Oct;56(4):574-81. doi: 10.1159/000126276.
The present study was designed to evaluate the immunological outcome resulting from experimental conditions involving different corticosterone and prolactin ratios in rats. One set of experiments was conducted to assess the effects of prolactin and corticosterone on the in vitro mitogen-induced proliferation of spleen lymphocytes from animals previously submitted to the manipulation of their glucocorticoid status throughout adrenalectomy (ADX) and/or exposure to acute stress. The results indicated that prolactin (5 x 10(-9) M) induced a significant increase in concanavalin A- (ConA) induced proliferation of splenocytes only from ADX-control, unstressed, rats. However, a lower dose of prolactin (10(-9) M) failed to influence lymphoproliferation. Corticosterone (2 x 10(-8) and 10(-7) M) induced a dose-dependent reduction in lymphocyte proliferation in all experimental groups. Further experiments were conducted to study the relative potency of prolactin to antagonize the in vitro corticosterone-induced suppression of ConA-stimulated lymphocytes. The results showed, on the one hand, that higher doses of prolactin (10(-8) and 5 x 10(-8) M) were effective in stimulating ConA-induced lymphocyte proliferation in control, undisturbed, rats. They also showed that when prolactin and corticosterone are simultaneously added to the cultures, the immunostimulatory effect induced by a dose of 10(-8) M of prolactin can either predominate over a weak suppressive action of corticosterone (2 x 10(-8) M) or totally antagonize to normal values a marked immunosuppression induced by a higher dose of corticosterone (10(-7) M). These data support the view that different ratios between prolactin and corticosterone concentrations can result in differential immunological outcome.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在评估大鼠体内不同皮质酮和催乳素比例的实验条件所产生的免疫结果。进行了一组实验，以评估催乳素和皮质酮对先前通过肾上腺切除术（ADX）和/或暴露于急性应激来操控其糖皮质激素状态的动物脾脏淋巴细胞体外有丝分裂原诱导增殖的影响。结果表明，催乳素（5×10⁻⁹ M）仅能显著增加来自ADX对照、未受应激的大鼠脾细胞的刀豆球蛋白A（ConA）诱导增殖。然而，较低剂量的催乳素（10⁻⁹ M）未能影响淋巴细胞增殖。皮质酮（2×10⁻⁸和10⁻⁷ M）在所有实验组中均诱导淋巴细胞增殖呈剂量依赖性降低。进一步的实验研究了催乳素拮抗体外皮质酮诱导的ConA刺激淋巴细胞抑制作用的相对效力。结果一方面显示，较高剂量的催乳素（10⁻⁸和5×10⁻⁸ M）能有效刺激对照、未受干扰大鼠的ConA诱导淋巴细胞增殖。这些结果还表明，当将催乳素和皮质酮同时添加到培养物中时，10⁻⁸ M剂量的催乳素所诱导的免疫刺激作用可要么在皮质酮（2×10⁻⁸ M）的微弱抑制作用之上占主导，要么完全拮抗较高剂量皮质酮（10⁻⁷ M）所诱导的明显免疫抑制，使其恢复到正常值。这些数据支持了这样一种观点，即催乳素和皮质酮浓度之间的不同比例可导致不同的免疫结果。（摘要截断于250字）
