Stefulj J, Cicin-Sain L, Schauenstein K, Jernej B
Laboratory of Neurochemistry and Molecular Neurobiology, Department of Molecular Genetics, Rudjer Boskovic Institute, Zagreb, Croatia.
Neuroimmunomodulation. 2001;9(2):103-8. doi: 10.1159/000049013.
The effect of serotonin (5-hydroxytryptamine; 5HT) on the in vitro proliferation of mitogen-stimulated lymphocytes was studied in primary cultures of rat spleen cells.
5HT was added to the cultures 1 h prior to the mitogen, at final concentrations from 10(-13) up to 10(-2) M. T and B cell mitogens (concanavalin A, pokeweed mitogen and lipopolysaccharide) were used at suboptimal and optimal concentrations. The cell proliferation was measured 24-72 h after the addition of mitogen. The effect of each 5HT concentration was studied on a group of 6-12 animals and was expressed as a percentage of the control values obtained with mitogen alone.
No significant effect of 5HT at concentrations from 10(-13) to 10(-5) M was found. At concentrations of > or =10(-4) M, a regular dose-dependent inhibition of the lymphocyte proliferation appeared, the concentration producing the half-maximal effect being 6 x 10(-4) M. The observed suppression was not due to 5HT cytotoxicity toward spleen cells.
With the experimental system used, we failed to confirm an immunostimulatory effect of 5HT in the range of concentrations of its receptor sensitivities or lower, but found a clear-cut immunoinhibitory effect at higher concentrations.
在大鼠脾细胞原代培养物中研究血清素（5-羟色胺；5HT）对丝裂原刺激的淋巴细胞体外增殖的影响。
在加入丝裂原前1小时向培养物中添加5HT，终浓度范围为10^(-13)至10^(-2)M。T细胞和B细胞丝裂原（刀豆球蛋白A、商陆丝裂原和脂多糖）使用次优和最佳浓度。在加入丝裂原后24至72小时测量细胞增殖。研究了每组6至12只动物中每种5HT浓度的影响，并表示为仅用丝裂原获得的对照值的百分比。
未发现浓度为10^(-13)至10^(-5)M的5HT有显著影响。在浓度≥10^(-4)M时，出现了对淋巴细胞增殖的规律剂量依赖性抑制，产生半数最大效应的浓度为6×10^(-4)M。观察到的抑制不是由于5HT对脾细胞的细胞毒性。
在所使用的实验系统中，我们未能证实在其受体敏感性浓度范围内或更低浓度时5HT的免疫刺激作用，但发现在较高浓度时有明确的免疫抑制作用。
