Hu S B, Tannahill L A, Lightman S L
Neuroendocrinology Unit, Charing Cross and Westminster Medical School, Charing Cross Hospital, London, UK.
Neuroendocrinology. 1992 Oct;56(4):591-6. doi: 10.1159/000126278.
Dexamethasone and aldosterone inhibit hypothalamic corticotropin releasing factor-41 (CRF) release. The possible receptors through which these adrenal steroids affect CRF release were studied using rat fetal hypothalamic cell cultures. Neither the antimineralocorticoid RU 28318 nor the antiglucocorticoid RU 38486 alone had any effect on forskolin-stimulated CRF release. RU 38486 and RU 28318 however suppressed dexamethasone (1 microM)- and aldosterone (1 microM)-induced inhibition of forskolin (20 microM)-stimulated CRF release, respectively, suggesting that dexamethasone and aldosterone reduce CRF release through type II and type I corticosteroid receptors, respectively. RU 38486 had no effect on aldosterone-induced inhibition of forskolin-stimulated CRF release, nor did RU 28318 have any effect on dexamethasone-induced inhibition of forskolin-stimulated CRF release, indicating specificity of the binding of aldosterone with type I receptors, and dexamethasone with type II receptors in the hypothalamic cell cultures.
地塞米松和醛固酮可抑制下丘脑促肾上腺皮质激素释放因子 -41（CRF）的释放。利用大鼠胎儿下丘脑细胞培养物研究了这些肾上腺类固醇影响CRF释放可能通过的受体。抗盐皮质激素RU 28318和抗糖皮质激素RU 38486单独使用时对福斯高林刺激的CRF释放均无任何影响。然而，RU 38486和RU 28318分别抑制了地塞米松（1微摩尔）和醛固酮（1微摩尔）诱导的对福斯高林（20微摩尔）刺激的CRF释放的抑制作用，这表明地塞米松和醛固酮分别通过II型和I型糖皮质激素受体减少CRF释放。RU 38486对醛固酮诱导的对福斯高林刺激的CRF释放的抑制作用无影响，RU 28318对地塞米松诱导的对福斯高林刺激的CRF释放的抑制作用也无影响，这表明在丘脑细胞培养物中醛固酮与I型受体结合以及地塞米松与II型受体结合具有特异性。
