Dall'Asta C, Galaverna G, Dossena A, Marchelli R
Dipartimento di Chimica Organica e Industriale, Università degli Studi di Parma, Parco Area delle Scienze 17/A, I-43100 Parma, Italy.
J Chromatogr A. 2004 Jan 23;1024(1-2):275-9. doi: 10.1016/j.chroma.2003.10.025.
In this paper, we propose a new, rapid, highly sensitive and reproducible RP-HPLC-FLD method for the detection of ochratoxin A (OTA) in wine, by directly injecting the liquid in the chromatographic system without any extraction or clean-up. An alkaline mobile phase (NH4Cl:CH-CN 85:15 (v/v), 20 mM, pH 9.8) was used to obtain a distinct fluorescence enhancement. This improvement allows to reach, without an immunoaffinity clean-up or concentration, a detection limit of 0.05 ng/ml, which is similar to those commonly obtained after immunoaffinity purification and acidic elution. The method was statistically validated and directly applied to a series of wine samples.
在本文中，我们提出了一种新的、快速、高灵敏度且可重现的反相高效液相色谱-荧光检测法（RP-HPLC-FLD），用于检测葡萄酒中的赭曲霉毒素A（OTA），该方法直接将液体注入色谱系统，无需任何萃取或净化步骤。使用碱性流动相（氯化铵：乙腈85:15（v/v），20 mM，pH 9.8）以获得明显的荧光增强。这种改进使得在无需免疫亲和净化或浓缩的情况下，检测限可达0.05 ng/ml，这与免疫亲和纯化和酸性洗脱后通常获得的检测限相似。该方法经过统计学验证，并直接应用于一系列葡萄酒样品。
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