Czerwiecki L, Wilczyńska G, Kwiecień A
Institute of Agricultural and Food Biotechnology, Warsaw, Poland.
Food Addit Contam. 2005 Feb;22(2):158-62. doi: 10.1080/02652030500038066.
A routine method appropriate for the determination of ochratoxin A (OTA) in wine, grape juice and grape juice drinks was described, and the occurrence of the mycotoxin was investigated in the most popular red wines, grape juice and grape juice drinks available on the Polish market. After clean-up on immunoaffinity column, samples were analysed by RP-HPLC using a fluorescence detector at 330 and 460 nm. The average OTA recoveries from spiked blank wine samples varied from 60 to 82%, and RSD% ranged from 5 to 14%. The OTA recovery for spiked grape juice and grape juice drinks were 80-100%, but the RSD% was between 7 and 10%. The limit of detection and limit of quantitation for all sample types were 0.5 and 2.0 ng l(-1), respectively. Fifty-three samples of red wine and seven samples of grape juice and grape drinks were assessed by means of this analytical procedure. OTA was detected in most wine samples (92%); its concentrations ranged from 2.2 to 6710 ng l(-1). In all grape juice and drink samples, OTA levels ranged from 1.6 to 64.7 ng l(-1).
描述了一种适用于测定葡萄酒、葡萄汁和葡萄汁饮料中赭曲霉毒素A（OTA）的常规方法，并对波兰市场上最受欢迎的红葡萄酒、葡萄汁和葡萄汁饮料中该霉菌毒素的存在情况进行了调查。在免疫亲和柱净化后，使用荧光检测器在330和460 nm波长下通过反相高效液相色谱法（RP-HPLC）对样品进行分析。加标空白葡萄酒样品的OTA平均回收率在60%至82%之间，相对标准偏差（RSD%）在5%至14%之间。加标葡萄汁和葡萄汁饮料的OTA回收率为80%-100%，但RSD%在7%至10%之间。所有样品类型的检测限和定量限分别为0.5和2.0 ng l(-1)。通过该分析程序对53个红葡萄酒样品、7个葡萄汁和葡萄饮料样品进行了评估。大多数葡萄酒样品（92%）中检测到OTA；其浓度范围为2.2至6710 ng l(-1)。在所有葡萄汁和饮料样品中，OTA水平在1.6至64.7 ng l(-1)之间。
