Otake S, Dee S A, Moon R D, Rossow K D, Trincado C, Pijoan C
Swine Disease Eradication Center, University of Minnesota College of Veterinary Medicine, 385 Animal Science/ Veterinary Medicine Building, 1988 Fitch Avenue, St Paul, MN 55108, USA.
Vet Rec. 2004 Jan 17;154(3):80-5. doi: 10.1136/vr.154.3.80.
The objectives of the study were to determine the site of porcine reproductive and respiratory syndrome virus (PRRSV) in individual houseflies, to assess whether an individual housefly could transmit PRRSV to a susceptible pig, and to compare the ability of PCR, virus isolation and a pig bioassay to detect PRRSV in houseflies. In the first experiment 26 houseflies were fed on a pig infected experimentally with PRRSV; 13 were processed as a whole fly homogenate, while an exterior surface wash and a gut homogenate were collected from the other 13. Infectious PRRSV was recovered from nine of the whole fly homogenates, 12 of the gut homogenates and one of the exterior surface washes. In the second experiment, two of 10 individual houseflies, which had fed on an infected pig, transmitted PRRSV to a susceptible pig in a controlled manual transmission protocol. In the third experiment, single flies or pools of 30 flies were immersed in different concentrations of a PRRSV inoculum, then tested by PCR, virus isolation and bioassay. The virus was detected at a concentration of 10(1) TCID50/ml by PCR, 10(2) TCID50/ml by the bioassay and 10(3) TCID50/ml by virus isolation.
该研究的目的是确定猪繁殖与呼吸综合征病毒（PRRSV）在单个家蝇体内的位置，评估单个家蝇是否能将PRRSV传播给易感猪，并比较聚合酶链反应（PCR）、病毒分离和猪生物测定法检测家蝇中PRRSV的能力。在第一个实验中，让26只家蝇取食经实验感染PRRSV的猪；13只作为完整家蝇匀浆处理，另外13只收集其体表冲洗液和肠道匀浆。从9份完整家蝇匀浆、12份肠道匀浆和1份体表冲洗液中分离出了具有传染性的PRRSV。在第二个实验中，在可控的人工传播实验方案中，10只取食感染猪的单个家蝇中有2只将PRRSV传播给了易感猪。在第三个实验中，将单只家蝇或30只家蝇的混合体浸入不同浓度的PRRSV接种物中，然后通过PCR、病毒分离和生物测定法进行检测。通过PCR检测到病毒的浓度为10（1）半数组织培养感染剂量（TCID50）/毫升，通过生物测定法为10（2）TCID50/毫升，通过病毒分离为10（3）TCID50/毫升。
