Ben-Ismail R, Smith D F, Ready P D, Ayadi A, Gramiccia M, Ben-Osman A, Ben-Rachid M S
Département de Parasitologie, Faculté de Médecine, Tunis, Tunisia.
Trans R Soc Trop Med Hyg. 1992 Sep-Oct;86(5):508-10. doi: 10.1016/0035-9203(92)90087-s.
A diagnostic kinetoplast deoxyribonucleic acid (DNA) probe has been used to identify as Leishmania infantum amastigotes taken from the lesions (in dermal fluid or lesion triturates) of 7 of 8 patients suffering from sporadic cutaneous leishmaniasis in north Tunisia. All 8 isolates were very difficult to culture but, subsequently, sufficient parasites of one isolate have been grown for isoenzyme typing, and were identified as zymodeme MON 24 of L. infantum. The advantages of using recombinant DNA probes for the identification of parasites from lesions are discussed.
一种诊断性动基体脱氧核糖核酸（DNA）探针已被用于鉴定从突尼斯北部8例散发性皮肤利什曼病患者中的7例病变部位（皮肤液或病变研磨物）采集的无鞭毛体为婴儿利什曼原虫。所有8个分离株都极难培养，但随后，已培养出一个分离株的足够寄生虫用于同工酶分型，并被鉴定为婴儿利什曼原虫的酶带型MON 24。讨论了使用重组DNA探针从病变中鉴定寄生虫的优点。
