Aoun K, Bouratbine A, Harrat Z, Guizani I, Mokni M, Bel Hadj Ali S, Ben Osman A, Belkaïd M, Dellagi K, Ben Ismaïl R
Laboratoire de parasitologie clinique, Institut Pasteur de Tunis, Tunisie.
Bull Soc Pathol Exot. 2000 Apr;93(2):101-3.
This study refers to 23 patients presenting with the sporadic forms of cutaneous leishmaniasis encountered in northern most humid parts of Tunisia. Culture inoculation for parasitic isolation was processed using two media: the classical NNN and a rabbit serum based medium (SLC). Cultures were positive in 17 cases with SLC medium and 13 cases with NNN medium. Eight isolates were typed using 15 isoenzymes systems. Six isolates were identified as Leishmania infantum MON-24 which confirms the crucial role of this zymodeme in causing this form of cutaneous leishmaniasis. The other two isolates were identified as Leishmania infantum MON-1, which is the principal agent of visceral leishmaniasis in the Mediterranean area.
本研究涉及23例在突尼斯最北部潮湿地区出现的散发性皮肤利什曼病患者。使用两种培养基进行寄生虫分离的培养接种:经典的NNN培养基和基于兔血清的培养基(SLC)。SLC培养基培养阳性17例,NNN培养基培养阳性13例。使用15种同工酶系统对8株分离株进行分型。6株分离株被鉴定为婴儿利什曼原虫MON-24,这证实了该酶型在引起这种皮肤利什曼病中的关键作用。另外两株分离株被鉴定为婴儿利什曼原虫MON-1,它是地中海地区内脏利什曼病的主要病原体。