水通道蛋白-1的表达在人和小鼠角膜内皮功能障碍中降低。
Aquaporin-1 expression is decreased in human and mouse corneal endothelial dysfunction.
作者信息
Macnamara Elizabeth, Sams G Walter, Smith Kimberly, Ambati Jayakrishna, Singh Nirbhai, Ambati Balamurali K
机构信息
Department of Ophthalmology, Medical College of Georgia, Augusta 30912, USA.
出版信息
Mol Vis. 2004 Jan 26;10:51-6.
PURPOSE
To determine if aquaporin-1 expression is decreased in human and mouse corneal endothelial dysfunction.
METHODS
Immunohistochemistry with anti-aquaporin 1 antibody and confocal microscopy were used to study a case series of human corneal specimens, and a mouse model of corneal endothelial injury was created with injection of sterile hot water into the anterior chamber with a 33 gauge needle. Western blotting of mouse and human corneas was used to provide confirmatory evidence.
RESULTS
Aquaporin-1 was found to be expressed in normal human cornea and decreased in human corneas with endothelial disease but not in human corneas with non-endothelial corneal disease. Aquaporin-1 was also found to be reduced in mouse corneas subjected to corneal endothelial injury. Both results were confirmed by western blot analysis.
CONCLUSIONS
Corneal endothelial injury is associated with decreased aquaporin-1 expression.
目的
确定水通道蛋白-1在人类和小鼠角膜内皮功能障碍中表达是否降低。
方法
使用抗水通道蛋白1抗体进行免疫组织化学和共聚焦显微镜检查，研究一系列人类角膜标本，并通过用33号针头向前房注射无菌热水建立小鼠角膜内皮损伤模型。采用小鼠和人类角膜的蛋白质免疫印迹法提供确证证据。
结果
发现水通道蛋白-1在正常人类角膜中表达，在患有内皮疾病的人类角膜中表达降低，但在患有非内皮角膜疾病的人类角膜中未降低。还发现水通道蛋白-1在遭受角膜内皮损伤的小鼠角膜中减少。两种结果均经蛋白质免疫印迹分析证实。
结论
角膜内皮损伤与水通道蛋白-1表达降低有关。
Zotero 插件