热休克预处理可防止东莨菪碱对大鼠海马CA1突触长时程增强的抑制作用。

Heat-shock pretreatment prevents suppression of long-term potentiation induced by scopolamine in rat hippocampal CA1 synapses.

作者信息

Lin Yi-Wen, Yang Hsiu-Wen, Min Ming-Yuan, Chiu Tsai-Hsien

机构信息

Department of Physiology, National Yang-Ming University, Taipei 112, Taiwan, ROC.

出版信息

Brain Res. 2004 Mar 5;999(2):222-6. doi: 10.1016/j.brainres.2003.11.057.

DOI:10.1016/j.brainres.2003.11.057

PMID:14759502

Abstract

We examined the effect of heat-shock pretreatment on long-term potentiation (LTP) in the CA1 hippocampal slices of the rat using the muscarinic blocker scopolamine as the LTP (memory) suppressor. Time course study using immunohistochemical techniques indicated peak expression of HSP70 16 h after heat-shock treatment. Focusing on that time point we found tetanic stimulation (at 100 Hz) induced LTP of 191.1+/-12.2% in control slices (n=7), which was suppressed by scopolamine to 114.5+/-2.8 %. Heat-shock pretreatment successfully prevented such suppression (216.6+/-38.2% and 190.2+/-10.6% with and without scopolamine, respectively, n=7). Both HSP expression and LTP responses were relatively small taken either 2 or 48 h after heat-shock or sham pretreatment. These results suggest that the induction of HSPs is time-dependent and can prevent scopolamine-mediated LTP suppression.

摘要

我们使用毒蕈碱阻断剂东莨菪碱作为长时程增强（LTP，记忆）抑制剂，研究了热休克预处理对大鼠海马CA1区脑片LTP的影响。采用免疫组化技术进行的时间进程研究表明，热休克处理后16小时HSP70表达达到峰值。聚焦于该时间点，我们发现强直刺激（100Hz）在对照脑片（n = 7）中诱导的LTP为191.1±12.2%，东莨菪碱将其抑制至114.5±2.8%。热休克预处理成功地防止了这种抑制（分别在有和没有东莨菪碱的情况下为216.6±38.2%和190.2±10.6%，n = 7）。热休克或假预处理后2小时或48小时，HSP表达和LTP反应都相对较小。这些结果表明，HSPs的诱导具有时间依赖性，并且可以防止东莨菪碱介导的LTP抑制。