Coll Josep, Tandrón Yudelsy
Instituto de Investigaciones Químicas y Ambientales de Barcelona Josep Pascual Vila, Consejo Superior de Investigaciones Científicas, Jordi Girona 18-26, 08034-Barcelona, Spain.
Phytochemistry. 2004 Feb;65(4):387-92. doi: 10.1016/j.phytochem.2003.09.018.
In addition to three out of the four previously reported neo-clerodanes already found in Teucrium fruticans (fruticolone, isofruticolone and 8 beta-hydroxyfruticolone), and 6-acetylteucjaponin B (isolated from T. scordium and T. grisebachii), four new neo-clerodanes, namely 7 beta-hydroxyfruticolone, 11-hydroxyfruticolone, deacetylfruticolone and 6-acetyl-10-hydroxyteucjaponin B were also isolated. The structures were unambiguously elucidated based on extensive NMR spectral studies (one- and two-dimensional experiments). Semi-prep-HPLC proved to be a convenient purification procedure, occasionally being followed by TLC. The new compounds were assayed against Spodoptera littoralis and two of them were shown to have potent antifeedant activity.
除了在穗花牡荆（穗花牡荆素、异穗花牡荆素和8β-羟基穗花牡荆素）中已发现的四种先前报道的新克罗烷类化合物中的三种，以及6-乙酰基条叶荆芥苷B（从蒜味香科科和灰毛香科科中分离得到）之外，还分离出了四种新的新克罗烷类化合物，即7β-羟基穗花牡荆素、11-羟基穗花牡荆素、去乙酰穗花牡荆素和6-乙酰基-10-羟基条叶荆芥苷B。基于广泛的核磁共振光谱研究（一维和二维实验），明确阐明了这些化合物的结构。半制备高效液相色谱被证明是一种方便的纯化方法，偶尔会继之以薄层色谱法。对新化合物进行了针对棉铃虫的测定，其中两种显示出有效的拒食活性。
