Davidson J, Ekramoddoullah A K
Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, British Columbia V8Z 1M5, Canada.
Tree Physiol. 1997 Oct;17(10):663-9. doi: 10.1093/treephys/17.10.663.
We compared bark proteins from four contrasting (blister rust-resistant versus susceptible) half-sib seedling pairs of western white pine (Pinus monticola D. Don). Pooled proteins from resistant and susceptible groups (four trees per group) were separated by two-dimensional gel electrophoresis, silver stained, and analyzed with the aid of a laser scanner interfaced with a computerized gel documentation system. Qualitative and quantitative protein differences were observed between resistant and susceptible groups. The number of proteins unique to a group was greater in the susceptible category than in the resistant category. Biosynthesis of some common proteins was enhanced near lesioned areas of susceptible seedlings. Many proteins shared similar charge and mass characteristics with those of pathogenesis-related (PR) proteins. Two protein bands were isolated and partially characterized by N-terminal amino acid sequencing: a 10.6-kDa band that was selectively enriched in all resistant individuals, and a 26.0-kDa band that was enriched in some susceptible individuals. The significance of these protein differences and the possible use of selected proteins as disease or resistance markers are discussed.
我们比较了西部白松（Pinus monticola D. Don）四个对比鲜明（抗疱锈病与易感疱锈病）的半同胞幼苗对的树皮蛋白质。将抗性组和易感组（每组四棵树）的混合蛋白质通过二维凝胶电泳进行分离，银染，并借助与计算机化凝胶成像系统相连的激光扫描仪进行分析。在抗性组和易感组之间观察到了蛋白质的定性和定量差异。易感组中特有的蛋白质数量比抗性组更多。在易感幼苗的损伤区域附近，一些常见蛋白质的生物合成增强。许多蛋白质与病程相关（PR）蛋白具有相似的电荷和质量特征。分离出两条蛋白带，并通过N端氨基酸测序进行了部分表征：一条10.6 kDa的带在所有抗性个体中选择性富集，一条26.0 kDa的带在一些易感个体中富集。讨论了这些蛋白质差异的意义以及所选蛋白质作为疾病或抗性标记的可能用途。
