Liu Xing-E, Sun Xiao-Dong, Wu Jin-Min
Center of Oncology, the Affiliated Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou 310016, Zhejiang Province, China.
World J Gastroenterol. 2004 Feb 1;10(3):352-5. doi: 10.3748/wjg.v10.i3.352.
To investigate the expression of pathological factors of VEGF-C and its receptor FLT-4 in primary gastric cancer and adjacent normal tissues.
The expression of VEGF-C and FLT-4 was studied in 80 primary gastric cancers and adjacent normal tissues from the same patients by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immumohistochemistry.
Both primary gastric cancer and adjacent normal tissue could express VEGF-C and FLT-4, and FLT-4 expression was also detected in endothelial cells of stromal blood vessels and lymphatic vessels. There was a significant difference in expression of VEGF-C between primary tumor and adjacent normal tissue samples (P=0.01), and a statistical correlation between VEGF-C and FLT-4 expression in tumors (P=0.00886). With regard to VEGF-C expression, there was a significant difference between moderate-poor differential type and high differential type (P=0.032), and a significant difference between positive and negative lymph node metastases (P=0.024). However, there was no significant difference between positive and negative serosal invasions (P=0.219).
VEGF-C and its receptor FLT-4 play a role in the development of gastric cancer, and the tumors with expression of VEGF-C and FLT-4 are more likely to have lymph node metastasis.
研究血管内皮生长因子C（VEGF-C）及其受体FLT-4在原发性胃癌及癌旁正常组织中病理因子的表达情况。
采用半定量逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，对80例原发性胃癌患者及其癌旁正常组织中VEGF-C和FLT-4的表达进行研究。
原发性胃癌组织和癌旁正常组织均能表达VEGF-C和FLT-4，在基质血管和淋巴管的内皮细胞中也检测到FLT-4表达。原发性肿瘤组织与癌旁正常组织样本中VEGF-C的表达存在显著差异（P = 0.01），肿瘤组织中VEGF-C与FLT-4的表达具有统计学相关性（P = 0.00886）。在VEGF-C表达方面，中低分化型与高分化型之间存在显著差异（P = 0.032），有淋巴结转移与无淋巴结转移之间存在显著差异（P = 0.024）。然而，浆膜侵犯阳性与阴性之间无显著差异（P = 0.219）。
VEGF-C及其受体FLT-4在胃癌发生发展过程中发挥作用，VEGF-C和FLT-4表达阳性的肿瘤更易发生淋巴结转移。
