Sun Xiao-Dong, Liu Xing-E, Wu Jin-Min, Cai Xiu-Jun, Mou Yi-Ping, Li Jun-Da
Department of General Surgery, the Affiliated Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou 310016, Zhejiang Province, China.
World J Gastroenterol. 2004 May 1;10(9):1382-5. doi: 10.3748/wjg.v10.i9.1382.
To investigate the expression and pathological factors of Angiopoietin-2 (Ang-2) in primary gastric cancers and adjacent normal tissues.
The expression of Angiopoietin-2 and VEGF were studied in 72 primary gastric cancers and adjacent normal tissues from the same patients by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry.
Ang-2 was mainly expression in tumor cells. There were significantly difference between expression of Ang-2 in primary gastric cancer and in adjacent normal tissue samples (P=0.003). It was statistically correlation between Ang-2 and VEGF expression in tumors (P=0.0055). With regard to Ang-2 expression in tumors, there were significant difference between early stage and advanced stage (P=0.017), and significant difference between positive vascular involvement and negative vascular involvement (P=0.032). However, there was no significant difference between moderate-poor differential type and high differential type (P=0.908), between positive lymph node metastasis and negative lymph node metastasis (P=0.752), between positive serosal invasion and negative serosal invasion (P=0.764). The cases with expression of Ang-2 were increasing with advanced stage and vascular involvement.
The results manifested that Angiopoietin-2, coordinated with VEGF, play role in regulating tumor angiogenesis of gastric cancer.
研究血管生成素-2（Ang-2）在原发性胃癌及癌旁正常组织中的表达情况及病理因素。
采用半定量逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，研究72例原发性胃癌及同一患者癌旁正常组织中血管生成素-2和血管内皮生长因子（VEGF）的表达。
Ang-2主要在肿瘤细胞中表达。原发性胃癌组织与癌旁正常组织中Ang-2的表达有显著差异（P=0.003）。肿瘤组织中Ang-2与VEGF的表达具有统计学相关性（P=0.0055）。就肿瘤组织中Ang-2的表达而言，早期与晚期有显著差异（P=0.017），血管受累阳性与阴性有显著差异（P=0.032）。然而，中低分化型与高分化型之间（P=0.908）、淋巴结转移阳性与阴性之间（P=0.752）、浆膜侵犯阳性与阴性之间（P=0.764）无显著差异。Ang-2表达阳性的病例随肿瘤分期进展和血管受累情况增加。
结果表明，血管生成素-2与VEGF协同作用，在胃癌肿瘤血管生成调节中发挥作用。
