Zhang Jun, Ge Sheng-xiang, Huang Guo-yong, Li Shao-wei, He Zhi-qiang, Wang Ying-bing, Zheng Ying-jie, Gu Ying, Ng Mun-hon, Xia Ning-shao
The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005, China.
Zhonghua Gan Zang Bing Za Zhi. 2004 Jan;12(1):7-10.
To evaluate the serological markers and biological marker in the diagnosis of hepatitis E infection in a rhesus monkey model.
86 rhesus monkeys had been infected with different doses of HEV. Hence, they were taken sequential blood samples at intervals up to 86 weeks for 4 hepatitis E virus (HEV) specific antibody assays (E2-IgM, E2-IgG, GL-IgG, and YES-IgG), and nucleic acid assay.
All the animals produced E2-IgG and all but one also produced E2-IgM and excreted the virus in stool, whereas positive rate of GL-IgG and YES IgG were low and correlated with virus level. Hepatitis occurred over a period of 4 weeks (between 3 an 7 weeks) after infection. Virological marker occurred mainly during incubation period and declined rapidly after onset of hepatitis. Seroconversion of E2-IgM occurred before onset of hepatitis in 70% monkeys and declined rapidly up to 50% of peak value after 4 weeks. E2-IgM seroconversion was closely paralleled by E2-IgG; however, E2-IgG persisted in all animals for the entire duration of experiment of up to 86 weeks. Production of GL-IgG and YES-IgG was delayed by one week after the E2 antibodies, these antibodies showed a transient occurrence and seroprevalence declined to 50% of the peak value over a period of 12 weeks.
E2-IgM might be used as a suitable acute hepatitis E marker, and E2-IgG as a suitable epidemiological marker. The seroconversion or titer elevation of GL-IgG and YES-IgG antibodies probably used to confirm the infection. The viral markers are optional for early diagnosis.
在恒河猴模型中评估血清学标志物和生物学标志物在戊型肝炎感染诊断中的作用。
86只恒河猴感染了不同剂量的戊型肝炎病毒。因此，在长达86周的时间里，每隔一段时间采集它们的血液样本，进行4种戊型肝炎病毒（HEV）特异性抗体检测（E2-IgM、E2-IgG、GL-IgG和YES-IgG）以及核酸检测。
所有动物均产生E2-IgG，除1只外其余动物均产生E2-IgM并在粪便中排出病毒，而GL-IgG和YES IgG的阳性率较低且与病毒水平相关。感染后4周（3至7周之间）出现肝炎。病毒学标志物主要出现在潜伏期，肝炎发病后迅速下降。70%的猴子在肝炎发病前出现E2-IgM血清学转换，4周后迅速下降至峰值的50%。E2-IgM血清学转换与E2-IgG密切平行；然而，E2-IgG在所有动物中在长达86周的整个实验期间持续存在。GL-IgG和YES-IgG的产生比E2抗体延迟1周，这些抗体呈短暂出现，血清阳性率在12周内下降至峰值的50%。
E2-IgM可能用作合适的急性戊型肝炎标志物，E2-IgG用作合适的流行病学标志物。GL-IgG和YES-IgG抗体的血清学转换或滴度升高可能用于确诊感染。病毒学标志物对早期诊断为可选项目。
