极低频磁场对HepG2细胞内游离钙的影响

[Effect of extremely low frequency magnetic fields on intracellular free calcium in HepG2 cells].

作者信息

Yang Wei, Xu Tao, Huo Xiao-lin, Song Tao

机构信息

Bioelectromagnetic Lab, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100080, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2003 Oct;21(5):332-4.

PMID:14761392

Abstract

OBJECTIVE

To study the effect of extremely low frequency magnetic fields on intracellular calcium concentration ([Ca(2+)]i).

METHODS

Fura-2 loaded HepG2 cells were exposed to 1.55 mT (average value), 16 Hz pulsed magnetic fields for 60 min and to 300 mT, 2 Hz rotating magnetic fields for 5 min, and then [Ca(2+)]i was measured by fluorescence spectrophotometer. [Ca(2+)]i of HepG2 cells was also measured when they were exposed to 0.9 mT [root mean square (rms)], 16 Hz sinusoidal magnetic fields in real time.

RESULTS

The R values (F(340) nm/F(380) nm) of the control and the exposed group were 2.4519 +/- 0.2378 and 2.5266 +/- 0.2915 respectively after HepG2 cells were exposed to 1.55 mT, 16 Hz magnetic fields, 1.365 0 +/- 0.0626 and 1.3602 +/- 0.0771 respectively to 300 mT, 2 Hz rotating magnetic fields. The ratios of the trendline slope [r((501 - 1,000)) / r((0 - 500))] from the data of R values were 1.1213 +/- 0.4559 and 1.0727 +/- 0.1971 respectively (P > 0.05), and the ratios of the intercept [b((501 - 1,000)) / b((0 - 500))] from the trendline were 0.9912 +/- 0.0098 and 0.9979 +/- 0.0060 (P > 0.05) when HepG2 cells were exposed to the 0.9 mT, 16 Hz sinusoidal magnetic fields.

CONCLUSION

The effect of extremely low frequency magnetic fields on [Ca(2+)]i of HepG2 cells under the experimental condition has not been found.

摘要

目的

研究极低频磁场对细胞内钙浓度（[Ca(2+)]i）的影响。

方法

将负载Fura-2的HepG2细胞暴露于1.55 mT（平均值）、16 Hz的脉冲磁场中60分钟，以及300 mT、2 Hz的旋转磁场中5分钟，然后用荧光分光光度计测量[Ca(2+)]i。当HepG2细胞实时暴露于0.9 mT[均方根（rms）]、16 Hz的正弦磁场时，也测量其[Ca(2+)]i。

结果

HepG2细胞暴露于1.55 mT、16 Hz磁场后，对照组和暴露组的R值（F(340)nm/F(380)nm）分别为2.4519±0.2378和2.5266±0.2915；暴露于300 mT、2 Hz旋转磁场后，分别为1.365 0±0.0626和1.3602±0.0771。R值数据的趋势线斜率[r((501 - 1,000)) / r((0 - 500))]之比分别为1.1213±0.4559和1.0727±0.1971（P>0.05）；当HepG2细胞暴露于0.9 mT、16 Hz正弦磁场时，趋势线的截距之比[b((501 - 1,000)) / b((0 - 500))]分别为0.9912±0.0098和0.9979±0.0060（P>0.05）。