枯草芽孢杆菌中编码单链DNA结合蛋白的两个旁系同源基因的差异表达。
Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein.
作者信息
Lindner Cordula, Nijland Reindert, van Hartskamp Mariska, Bron Sierd, Hamoen Leendert W, Kuipers Oscar P
机构信息
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, NL-9751 NN Haren, The Netherlands.
出版信息
J Bacteriol. 2004 Feb;186(4):1097-105. doi: 10.1128/JB.186.4.1097-1105.2004.
Abstract
The Bacillus subtilis genome comprises two paralogous single-stranded DNA binding protein (SSB) genes, ssb and ywpH, which show distinct expression patterns. The main ssb gene is strongly expressed during exponential growth and is coregulated with genes encoding the ribosomal proteins S6 and S18. The gene organization rpsF-ssb-rpsR as observed in B. subtilis is found in many gram-positive as well as some gram-negative bacteria, but not in Escherichia coli. The ssb gene is essential for cell viability, and like other SSBs its expression is elevated during SOS response. In contrast, the paralogous ywpH gene is transcribed from its own promoter at the onset of stationary phase in minimal medium only. Its expression is ComK dependent and its gene product is required for optimal natural transformation.
摘要
枯草芽孢杆菌基因组包含两个同源的单链DNA结合蛋白（SSB）基因，即ssb和ywpH，它们表现出不同的表达模式。主要的ssb基因在指数生长期强烈表达，并与编码核糖体蛋白S6和S18的基因共同调控。在枯草芽孢杆菌中观察到的基因组织rpsF - ssb - rpsR在许多革兰氏阳性菌以及一些革兰氏阴性菌中都存在，但在大肠杆菌中不存在。ssb基因对细胞活力至关重要，并且与其他SSB一样，其表达在SOS反应期间会升高。相比之下，同源的ywpH基因仅在基本培养基中稳定期开始时从其自身启动子转录。其表达依赖于ComK，并且其基因产物是最佳自然转化所必需的。
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