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Benzoate decreases the binding of cis,cis-muconate to the BenM regulator despite the synergistic effect of both compounds on transcriptional activation.尽管这两种化合物对转录激活有协同作用，但苯甲酸盐会降低顺式，顺式粘康酸与BenM调节因子的结合。
J Bacteriol. 2004 Feb;186(4):1200-4. doi: 10.1128/JB.186.4.1200-1204.2004.
2
Distinct effector-binding sites enable synergistic transcriptional activation by BenM, a LysR-type regulator.不同的效应物结合位点可实现由LysR型调控因子BenM介导的协同转录激活。
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Full-length structures of BenM and two variants reveal different oligomerization schemes for LysR-type transcriptional regulators.BenM 和两种变体的全长结构揭示了 LysR 型转录调节因子的不同寡聚化方案。
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Regulation of benzoate degradation in Acinetobacter sp. strain ADP1 by BenM, a LysR-type transcriptional activator.赖氨酸R型转录激活因子BenM对不动杆菌属ADP1菌株中苯甲酸降解的调控
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Generation of a catR deficient mutant of P. putida KT2440 that produces cis, cis-muconate from benzoate at high rate and yield.生成一个 catR 缺陷型突变的 P. putida KT2440，该突变体能以高速度和高产量从苯甲酸生产顺式，顺式-粘康酸。
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Curr Microbiol. 2008 Dec;57(6):609-14. doi: 10.1007/s00284-008-9251-4. Epub 2008 Sep 10.

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Oligomerization of BenM, a LysR-type transcriptional regulator: structural basis for the aggregation of proteins in this family.LysR 型转录调节因子 BenM 的寡聚化：该家族蛋白质聚集的结构基础。
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CatM regulation of the benABCDE operon: functional divergence of two LysR-type paralogs in Acinetobacter baylyi ADP1.拜氏不动杆菌ADP1中CatM对benABCDE操纵子的调控：两个LysR型旁系同源物的功能差异
Appl Environ Microbiol. 2006 Mar;72(3):1749-58. doi: 10.1128/AEM.72.3.1749-1758.2006.

本文引用的文献

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Crystallization of the effector-binding domains of BenM and CatM, LysR-type transcriptional regulators from Acinetobacter sp. ADP1.不动杆菌属ADP1菌株中LysR型转录调节因子BenM和CatM效应物结合结构域的结晶
Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):105-8. doi: 10.1107/s0907444903021589. Epub 2003 Dec 18.
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Crystal structure of a full-length LysR-type transcriptional regulator, CbnR: unusual combination of two subunit forms and molecular bases for causing and changing DNA bend.全长LysR型转录调节因子CbnR的晶体结构：两种亚基形式的异常组合以及引起和改变DNA弯曲的分子基础。
J Mol Biol. 2003 May 2;328(3):555-66. doi: 10.1016/s0022-2836(03)00312-7.
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Synergistic transcriptional activation by one regulatory protein in response to two metabolites.一种调节蛋白对两种代谢物产生协同转录激活作用。
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The chlorocatechol degradative genes, tfdT-CDEF, of Burkholderia sp. strain NK8 are involved in chlorobenzoate degradation and induced by chlorobenzoates and chlorocatechols.伯克霍尔德氏菌属NK8菌株的氯儿茶酚降解基因tfdT - CDEF参与氯苯甲酸酯的降解，并受氯苯甲酸酯和氯儿茶酚诱导。
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The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates.伯克霍尔德氏菌属NK8菌株中参与氯苯甲酸酯分解代谢的氯苯甲酸双加氧酶基因。
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Transcriptional activation of the chlorocatechol degradative genes of Ralstonia eutropha NH9.真养产碱菌NH9的氯儿茶酚降解基因的转录激活
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Transcriptional activation of the catechol and chlorocatechol operons: variations on a theme.儿茶酚和氯儿茶酚操纵子的转录激活：同一主题的变体
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Regulation of benzoate degradation in Acinetobacter sp. strain ADP1 by BenM, a LysR-type transcriptional activator.赖氨酸R型转录激活因子BenM对不动杆菌属ADP1菌株中苯甲酸降解的调控
J Bacteriol. 1998 May;180(9):2493-501. doi: 10.1128/JB.180.9.2493-2501.1998.
10
The structure of the cofactor-binding fragment of the LysR family member, CysB: a familiar fold with a surprising subunit arrangement.赖氨酰阻遏物家族成员CysB的辅因子结合片段的结构：具有惊人亚基排列的常见折叠结构。
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尽管这两种化合物对转录激活有协同作用，但苯甲酸盐会降低顺式，顺式粘康酸与BenM调节因子的结合。

Benzoate decreases the binding of cis,cis-muconate to the BenM regulator despite the synergistic effect of both compounds on transcriptional activation.

作者信息

Clark Todd J, Phillips Robert S, Bundy Becky M, Momany Cory, Neidle Ellen L

机构信息

Departments of Microbiology. Chemistry, and Pharmaceutical. Biomedical Sciences, University of Georgia, Athens, Georgia 30602.

出版信息

J Bacteriol. 2004 Feb;186(4):1200-4. doi: 10.1128/JB.186.4.1200-1204.2004.

DOI:10.1128/JB.186.4.1200-1204.2004
PMID:14762017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344200/
Abstract

Fluorescence emission spectroscopy was used to investigate interactions between two effectors and BenM, a transcriptional regulator of benzoate catabolism. BenM had a higher affinity for cis,cis-muconate than for benzoate as the sole effector. However, the presence of benzoate increased the apparent dissociation constant (reduced the affinity) of the protein for cis,cis-muconate. Similar results were obtained with truncated BenM lacking the DNA-binding domain. High-level transcriptional activation may require that some monomers within a BenM tetramer bind benzoate and others bind cis,cis-muconate.

摘要

荧光发射光谱法被用于研究两种效应物与苯甲酸酯分解代谢的转录调节因子BenM之间的相互作用。作为唯一的效应物，BenM对顺,顺-粘康酸的亲和力高于对苯甲酸酯的亲和力。然而，苯甲酸酯的存在增加了蛋白质对顺,顺-粘康酸的表观解离常数（降低了亲和力）。对于缺乏DNA结合结构域的截短型BenM也获得了类似的结果。高水平的转录激活可能要求BenM四聚体内的一些单体结合苯甲酸酯，而其他单体结合顺,顺-粘康酸。