Oct-1基因缺陷小鼠中的B细胞发育与免疫球蛋白转录
B cell development and immunoglobulin transcription in Oct-1-deficient mice.
作者信息
Wang Victoria E H, Tantin Dean, Chen Jianzhu, Sharp Phillip A
机构信息
Department of Biology and Center for Cancer Research and McGovern Institute, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307, USA.
出版信息
Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):2005-10. doi: 10.1073/pnas.0307304101. Epub 2004 Feb 4.
Abstract
The POU domain transcription factors Oct-1 and Oct-2 interact with the octamer element, a motif conserved within Ig promoters and enhancers, and mediate transcription from the Ig loci. Inactivation of Oct-2 by gene targeting results in normal B cell development and Ig transcription. To study the role of Oct-1 in these processes, the lymphoid compartment of RAG-1(-/-) animals was reconstituted with Oct-1-deficient fetal liver hematopoietic cells. Recipient mice develop B cells with levels of surface Ig expression comparable with wild type, although at slightly reduced numbers. These B cells transcribe Ig normally, respond to antigenic stimulation, undergo class switching, and use a normal repertoire of light chain variable segments. However, recipient mice show slight reductions in serum IgM and IgA. Thus, the Oct-1 protein is dispensable for B cell development and Ig transcription.
摘要
POU结构域转录因子Oct-1和Oct-2与八聚体元件相互作用，八聚体元件是免疫球蛋白（Ig）启动子和增强子中保守的基序，并介导Ig基因座的转录。通过基因靶向使Oct-2失活会导致正常的B细胞发育和Ig转录。为了研究Oct-1在这些过程中的作用，用缺乏Oct-1的胎肝造血细胞重建了RAG-1（-/-）动物的淋巴区室。受体小鼠发育出表面Ig表达水平与野生型相当的B细胞，尽管数量略有减少。这些B细胞正常转录Ig，对抗原刺激作出反应，进行类别转换，并使用正常的轻链可变区库。然而，受体小鼠的血清IgM和IgA略有降低。因此，Oct-1蛋白对于B细胞发育和Ig转录是可有可无的。
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