Hoffer Michael E, Allen Keith, Gottshall Kim, Moore Robert, Kopke Richard D, Wester Derin, Balaban Carey
Department of Defense Spatial Orientation Center, Department of Otolaryngology, Naval Medical Center San Diego, San Diego, CA 92134-2200, USA.
Int Tinnitus J. 2002;8(1):27-9.
Transtympanic gentamicin administration has become a popular modality in the treatment of Ménière's disease. This modality and other inner-ear medical therapy are gaining increased clinical and scientific attention. We previously described the kinetics and effects of gentamicin uptake into the inner ear after delivery of the medicine into the middle ear using a variety of different techniques and sustained-release modalities [1]. In our previous work, we reported an early peak perilymph concentration and the presence of intracellular gentamicin at the 4-hour time point. We also demonstrated the activation of inner-ear damage pathways at this early time point. In this report, we examine the kinetics of gentamicin at very early time points, 1 and 2 hours after administration. Healthy adult chinchillas underwent implantation of middle-ear sustained-release devices (one to each ear) containing gentamicin. The animals then were maintained in a neutral position and underwent perilymph gentamicin sampling at the two predetermined time points. This technique allowed us to assess accurately very early time point inner-ear gentamicin kinetics and to compare the activity. The samples then were run for concentration using mass spectrometry. The information gained from this study may increase our scientific understanding about the effects of gentamicin on the inner ear and may allow clinicians to treat patients more effectively for inner-ear disorders.
鼓室内注射庆大霉素已成为治疗梅尼埃病的一种常用方法。这种方法及其他内耳药物治疗正受到越来越多的临床和科学关注。我们之前使用多种不同技术和缓释方式，描述了将药物注入中耳后庆大霉素在内耳的摄取动力学及效应[1]。在我们之前的研究中，我们报告了给药后4小时外淋巴液浓度出现早期峰值以及细胞内存在庆大霉素。我们还证明了在此早期时间点内耳损伤途径被激活。在本报告中，我们研究给药后1小时和2小时这两个非常早期时间点的庆大霉素动力学。健康成年龙猫接受含有庆大霉素的中耳缓释装置植入（每只耳朵植入一个）。然后将动物保持在中立位置，并在两个预定时间点采集外淋巴液中的庆大霉素样本。该技术使我们能够准确评估非常早期时间点内耳庆大霉素的动力学并比较其活性。然后使用质谱法对样本进行浓度检测。从这项研究中获得的信息可能会增进我们对庆大霉素对内耳影响的科学理解，并可能使临床医生更有效地治疗内耳疾病患者。
