Hoffer M E, Balough B J, Kopke R D, Henderson J, DeCicco M, Wester D C, O'Leary M J, Balaban C
Department of Defense Spatial Orientation Center, Naval Medical Center San Diego, CA 92134-5000, USA.
Otolaryngol Head Neck Surg. 1999 May;120(5):643-8. doi: 10.1053/hn.1999.v120.a91762.
The use of transtympanic gentamicin has become a popular method of treating Meniere's disease; nevertheless, many questions still remain regarding this therapy. Until investigators can control the exact amount of medicine that is administered to the ear and have an understanding of the kinetics of gentamicin, therapy will continue to rely on empirical data. Previously we described the use of a fibrin-based sustained-release vehicle impregnated with gentamicin in the middle ears of chinchillas. With this model a kinetics curve of gentamicin was defined. The inner ears of these animals were submitted for immunohistochemical and histologic analysis. We discuss the ultrastructural changes seen and correlate this to our kinetics data. We also examine measurement of hair cell damage with heat shock protein levels. By better understanding the actions of gentamicin in this animal model, we hope to facilitate safer use of intratympanic medicines in our patient population.
经鼓膜使用庆大霉素已成为治疗梅尼埃病的一种常用方法；然而，关于这种疗法仍存在许多问题。在研究人员能够控制给予耳朵的精确药物量并了解庆大霉素的动力学之前，治疗将继续依赖经验数据。此前我们描述了在龙猫中耳使用浸渍有庆大霉素的纤维蛋白基缓释载体。利用该模型定义了庆大霉素的动力学曲线。对这些动物的内耳进行了免疫组织化学和组织学分析。我们讨论了观察到的超微结构变化，并将其与我们的动力学数据相关联。我们还通过热休克蛋白水平检测毛细胞损伤情况。通过更好地理解庆大霉素在该动物模型中的作用，我们希望在我们的患者群体中促进鼓室内药物的更安全使用。
