N,O-双（三甲基硅基）三氟乙酰胺和N-（叔丁基二甲基硅基）-N-甲基三氟乙酰胺作为气相色谱-质谱法测定雌激素雌酮和17α-乙炔雌二醇的衍生化试剂的适用性。
Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as derivatization reagents for the determination of the estrogens estrone and 17alpha-ethinylestradiol by gas chromatography-mass spectrometry.
作者信息
Shareef Ali, Parnis Craig J, Angove Michael J, Wells John D, Johnson Bruce B
机构信息
Colloid and Environmental Chemistry Laboratory, La Trobe University, PO Box 199, Bendigo, Vic. 3552, Australia.
出版信息
J Chromatogr A. 2004 Feb 13;1026(1-2):295-300. doi: 10.1016/j.chroma.2003.10.110.
This paper describes apreviously unreported problem with the use of N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA) and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) to derivatise the natural hormone estrone (E1) and the synthetic estrogen 17alpha-ethinylestradiol (EE2). The resulting trimethylsilyl (TMS) and t-butyldimethylsilyl (TBS) derivatives of EE2 were partially converted to their respective El derivatives. Therefore, these reagents may not be suitable for simultaneous determination of estrogens in environmental samples, which raises questions about the reliability of results from some earlier studies.
本文描述了使用N,O-双（三甲基硅基）三氟乙酰胺（BSTFA）和N-（叔丁基二甲基硅基）-N-甲基三氟乙酰胺（MTBSTFA）对天然激素雌酮（E1）和合成雌激素17α-乙炔雌二醇（EE2）进行衍生化时一个此前未报道的问题。EE2生成的三甲基硅基（TMS）和叔丁基二甲基硅基（TBS）衍生物部分转化为了各自的E1衍生物。因此，这些试剂可能不适用于环境样品中雌激素的同时测定，这对一些早期研究结果的可靠性提出了质疑。
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