Joo H J, Oh D K, Kim Y S, Lee K B, Kim S J
Department of Pathology, Ajou University School of Medicine, Suwon, Korea.
BJU Int. 2004 Feb;93(3):291-6. doi: 10.1111/j.1464-410x.2004.04604.x.
To investigate the relationship of caveolin-1 expression and microvessel density (MVD), a reflection of angiogenesis, with metastasis and prognosis in patients with clear cell renal cell carcinoma (RCC).
Formalin-fixed, paraffin-embedded tissue sections of clear cell RCC from 67 patients who had undergone radical nephrectomy were stained immunohistochemically with specific antibodies against caveolin-1 and CD34. Caveolin-1 immunostaining was semi-quantitatively estimated based on the proportion (percentage of positive cells) and intensity. MVD was determined with CD34-stained slides. The expression pattern of caveolin-1 and MVD was compared with the clinicopathological variables.
Eighteen patients had either synchronous or metachronous metastases and 11 died during the follow-up. Caveolin-1 intensity was significantly correlated with tumour size (P = 0.005), TNM stage (P = 0.028), M stage (P = 0.012), grade (P = 0.015), and metastasis (synchronous or metachronous; P < 0.001). The caveolin-1 proportion (P = 0.037) and MVD (P = 0.011) were significantly correlated with metastasis. MVD was correlated with caveolin-1 intensity (r = 0.385, P = 0.001) and caveolin-1 proportion (r = 0.388, P = 0.001). There was no difference in the expression of caveolin-1 and MVD between primary and metastatic sites. The survival of patients with higher caveolin-1 intensity was significantly worse than that of patients with lower caveolin-1 intensity. Multivariate analyses indicated that only M-stage was an independent prognostic factor for cancer-specific survival and caveolin-1 expression was not an independent factor.
Increased expression of caveolin-1 and MVD is associated with metastasis and a worse prognosis in clear cell RCC. Caveolin-1 expression is correlated with MVD. These results suggest that caveolin-1 may be important in the progression of clear cell RCC and angiogenesis may be affected by caveolin-1 during the progression of RCC.
探讨小窝蛋白-1表达与微血管密度（MVD，反映血管生成情况）在透明细胞肾细胞癌（RCC）患者中与转移及预后的关系。
对67例行根治性肾切除术患者的透明细胞RCC的福尔马林固定、石蜡包埋组织切片，用抗小窝蛋白-1和CD34的特异性抗体进行免疫组化染色。基于比例（阳性细胞百分比）和强度对小窝蛋白-1免疫染色进行半定量评估。用CD34染色切片测定MVD。将小窝蛋白-1的表达模式和MVD与临床病理变量进行比较。
18例患者有同步或异时转移，11例在随访期间死亡。小窝蛋白-1强度与肿瘤大小（P = 0.005）、TNM分期（P = 0.028）、M分期（P = 0.012）、分级（P = 0.015）及转移（同步或异时；P < 0.001）显著相关。小窝蛋白-1比例（P = 0.037）和MVD（P = 0.011）与转移显著相关。MVD与小窝蛋白-1强度（r = 0.385，P = 0.001）和小窝蛋白-1比例（r = 0.388，P = 0.001）相关。原发灶和转移灶之间小窝蛋白-1和MVD的表达无差异。小窝蛋白-1强度较高患者的生存率显著低于小窝蛋白-1强度较低的患者。多因素分析表明，只有M分期是癌症特异性生存的独立预后因素，小窝蛋白-1表达不是独立因素。
小窝蛋白-1和MVD表达增加与透明细胞RCC的转移及较差预后相关。小窝蛋白-1表达与MVD相关。这些结果表明，小窝蛋白-1在透明细胞RCC进展中可能很重要，且在RCC进展过程中血管生成可能受小窝蛋白-1影响。
