Yang Yingnan, Zhang Zhenya, Lu Jun, Maekawa Takaaki
Biomass Research Group, Institute for Energy Utilization, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8569, Japan.
Bioresour Technol. 2004 May;92(3):285-90. doi: 10.1016/j.biortech.2003.09.011.
A fixed-bed reactor with acclimated methanogens immobilized on a loofah support was studied on a laboratory scale to evaluate the system producing methane from the mixture of CO(2) and H(2) gas, with the production of vitamin B(12) as a by-product. Fermentation using CO(2)/H(2) acclimated methanogens was conducted in a jar fermentor with hydraulic retention times (HRTs) of three and six days. The performance of the reactor was mainly dependent on the HRT. With an HRT of three days, the methane production rate and the vitamin B(12) concentration in the culture broth were 6.18 l/l-reactor/h and 2.88 mg/l-culture liquid; these values were 11.96 l/l-reactor/h and 37.54 mg/l-culture liquid for an HRT of six days. A higher total cell mass of methanogens retained 42.5 g dry cell/l-culture liquid was achieved in the HRT of six days. The loofah carrier immobilized almost 95% of the methanogens, which led to a more effective bio-reaction. It was also observed that the fermentation system had a better ability to buffer pH, especially for an HRT of six days.
在实验室规模下研究了一种固定床反应器，该反应器将驯化的产甲烷菌固定在丝瓜载体上，以评估由二氧化碳和氢气混合物生产甲烷并副产维生素B12的系统。使用二氧化碳/氢气驯化的产甲烷菌在水力停留时间（HRT）为3天和6天的罐式发酵罐中进行发酵。反应器的性能主要取决于水力停留时间。水力停留时间为3天时，甲烷产率和培养液中维生素B12浓度分别为6.18升/反应器/小时和2.88毫克/升培养液；水力停留时间为6天时，这些值分别为11.96升/反应器/小时和37.54毫克/升培养液。在水力停留时间为6天时，产甲烷菌的总细胞质量更高，达到42.5克干细胞/升培养液。丝瓜载体固定了近95%的产甲烷菌，从而实现了更有效的生物反应。还观察到发酵系统具有更好的pH缓冲能力，特别是在水力停留时间为6天时。
