通过时间蛋白质组学揭示的海洋甲藻多环舌甲藻表达蛋白中的生物节律性。

Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics.

作者信息

Akimoto Hidetoshi, Wu Chun, Kinumi Tomoya, Ohmiya Yoshihiro

机构信息

Light and Control Research Area, PRESTO, Japan Science and Technology Corporation, 1-3-1 Kashiwadaiminami, Chitose, Hokkaido, Japan.

出版信息

Biochem Biophys Res Commun. 2004 Mar 5;315(2):306-12. doi: 10.1016/j.bbrc.2004.01.054.

DOI:10.1016/j.bbrc.2004.01.054

PMID:14766208

Abstract

The dinoflagellate Lingulodinium polyedrum shows circadian expression of proteins. Analysis of the expression profile of ca. 900 proteins (pI range 5-8; MW 22,000-120,000) by chronological proteomics in 12:12h light-dark ('day-night') cycles revealed 28 with quantitative circadian changes. The patterns were classified into three types: Phase 1 ('evening'), Phase 2 ('night'), and Phase 3 ('midnight'). Peptide mass fingerprinting identified luciferin-binding protein in Phase 2 and two components of the tricarboxylic acid cycle in Phases 1 and 2. Thus, rhythmicity appears to be related to physiological states and to control the metabolic pathway in L. polyedrum.

摘要

海洋甲藻多面舌甲藻显示出蛋白质的昼夜节律表达。通过时间蛋白质组学对约900种蛋白质（等电点范围为5-8；分子量为22,000-120,000）在12:12小时明暗（“昼夜”）循环中的表达谱进行分析，发现有28种蛋白质存在定量的昼夜变化。这些模式被分为三种类型：第1阶段（“傍晚”）、第2阶段（“夜间”）和第3阶段（“午夜”）。肽质量指纹图谱鉴定出第2阶段的荧光素结合蛋白以及第1阶段和第2阶段三羧酸循环的两个组分。因此，节律性似乎与生理状态相关，并控制多面舌甲藻的代谢途径。