Mittag M, Lee D H, Hastings J W
Department of Cellular and Molecular Biology, Harvard University, Cambridge, MA 02138.
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5257-61. doi: 10.1073/pnas.91.12.5257.
The circadian-expressed luciferin-binding protein from the dinoflagellate Gonyaulax polyedra is regulated at the translational level. We detected a protein, apparently a dimer, that binds specifically to the 3' untranslated region of its mRNA. Its binding site was localized within a 22-nt region in the 3' untranslated region containing seven UG repeats. The binding activity of this protein cycles on a daily basis, decreasing at the beginning of the night when synthesis of luciferin-binding protein starts and increasing at the end of the night when synthesis of luciferin-binding protein stops. This suggests that it functions as a clock-controlled repressor, preventing the translation of lbp mRNA during the day.
来自多甲藻的昼夜节律表达的荧光素结合蛋白在翻译水平受到调控。我们检测到一种蛋白质，显然是一种二聚体，它能特异性结合其mRNA的3'非翻译区。其结合位点位于3'非翻译区一个22个核苷酸的区域内，该区域包含七个UG重复序列。这种蛋白质的结合活性每天循环变化，在荧光素结合蛋白合成开始的夜晚开始时降低，在荧光素结合蛋白合成停止的夜晚结束时增加。这表明它作为一种生物钟控制的阻遏物发挥作用，在白天阻止lbp mRNA的翻译。
