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Antimicrobial properties of milk: dependence on presence of xanthine oxidase and nitrite.牛奶的抗菌特性：取决于黄嘌呤氧化酶和亚硝酸盐的存在。
Antimicrob Agents Chemother. 2002 Oct;46(10):3308-10. doi: 10.1128/AAC.46.10.3308-3310.2002.
2
Detection of traces of tetracyclines from fish with a bioluminescent sensor strain incorporating bacterial luciferase reporter genes.
J Agric Food Chem. 2002 Aug 14;50(17):4812-5. doi: 10.1021/jf020402l.
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An Escherichia coli biosensor strain for amplified and high throughput detection of antimicrobial agents.
J Biomol Screen. 2002 Apr;7(2):119-25. doi: 10.1177/108705710200700204.
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Expression of lux genes in a clinical isolate of Streptococcus pneumoniae: using bioluminescence to monitor gemifloxacin activity.肺炎链球菌临床分离株中lux基因的表达：利用生物发光监测吉米沙星活性。
Antimicrob Agents Chemother. 2002 Feb;46(2):538-42. doi: 10.1128/AAC.46.2.538-542.2002.
5
Validation of a noninvasive, real-time imaging technology using bioluminescent Escherichia coli in the neutropenic mouse thigh model of infection.在中性粒细胞减少小鼠大腿感染模型中使用生物发光大肠杆菌对一种非侵入性实时成像技术进行验证。
Antimicrob Agents Chemother. 2001 Jan;45(1):129-37. doi: 10.1128/AAC.45.1.129-137.2001.
6
Current methodologies for the analysis of aminoglycosides.氨基糖苷类分析的当前方法。
J Chromatogr B Biomed Sci Appl. 2000 Sep 29;747(1-2):69-93. doi: 10.1016/s0378-4347(00)00133-x.
7
Detection of tetracyclines with luminescent bacterial strains.利用发光细菌菌株检测四环素。
Luminescence. 2000 Sep-Oct;15(5):291-7. doi: 10.1002/1522-7243(200009/10)15:5<291::AID-BIO596>3.0.CO;2-B.
8
A group-specific microbiological test for the detection of tetracycline residues in raw milk.一种用于检测生乳中四环素残留的组特异性微生物学检测方法。
J Agric Food Chem. 2000 Aug;48(8):3372-7. doi: 10.1021/jf9911794.
9
A nisin bioassay based on bioluminescence.一种基于生物发光的乳链菌肽生物测定法。
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10
Use of a clinical Escherichia coli isolate expressing lux genes to study the antimicrobial pharmacodynamics of moxifloxacin.利用一株表达lux基因的临床大肠杆菌分离株研究莫西沙星的抗菌药效学。
J Antimicrob Chemother. 1999 Jun;43(6):829-32. doi: 10.1093/jac/43.6.829.

利用发光大肠杆菌和免疫血清特异性测定抗生素的新方法。

New approach for specific determination of antibiotics by use of luminescent Escherichia coli and immune serum.

作者信息

Vlasova Irina I, Asrieli Tatyana V, Gavrilova Elisaveta M, Danilov Vadim S

机构信息

Department of Microbiology, Faculty of Biology, M. V. Lomonosov Moscow State University, 119899 Moscow, Russia.

出版信息

Appl Environ Microbiol. 2004 Feb;70(2):1245-8. doi: 10.1128/AEM.70.2.1245-1248.2004.

DOI:10.1128/AEM.70.2.1245-1248.2004
PMID:14766615
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348806/
Abstract

This paper describes a possible application of luminescent Escherichia coli activated by blood serum for high-sensitivity and high-specificity assays of antibiotics in solutions. Antibiotics inhibited luminescence of a genetically engineered E. coli strain; the system sensitivity to some antibiotics grew notably after the cells had been preactivated by blood serum. The highest level of sensitivity (2.8 +/- 0.6 ng/ml) of luminescent cells was obtained for aminoglycoside antibiotics (gentamicin and streptomycin). It is feasible to create the specific biosensor for antibiotics on the basis of bioluminescent E. coli strains by applying sera containing antibodies against the antibiotic under assay. The presence of antibodies specific for gentamicin in serum affects inhibition of luminescent cells by gentamicin but not inhibition by other antibiotics.

摘要

本文描述了一种利用血清激活的发光大肠杆菌对溶液中的抗生素进行高灵敏度和高特异性检测的可能应用。抗生素会抑制基因工程改造的大肠杆菌菌株的发光；在细胞经血清预激活后，该系统对某些抗生素的敏感性显著提高。对于氨基糖苷类抗生素（庆大霉素和链霉素），发光细胞获得了最高灵敏度水平（2.8±0.6 ng/ml）。通过应用含有针对被测抗生素抗体的血清，基于生物发光大肠杆菌菌株创建抗生素特异性生物传感器是可行的。血清中庆大霉素特异性抗体的存在会影响庆大霉素对发光细胞的抑制作用，但不影响其他抗生素的抑制作用。