Page N A, Steele A D
MRC Medunsa Diarrhoeal Pathogens Research Unit, Medical University of Southern Africa, Medunsa 0204, South Africa.
J Clin Microbiol. 2004 Feb;42(2):595-600. doi: 10.1128/JCM.42.2.595-600.2004.
Serotype G2 rotavirus strains were isolated in seven countries on the African continent during 1999 and 2000. To investigate the associated DS-1 genogroup characteristics, subgroup (VP6) enzyme-linked immunosorbent assay, polyacrylamide gel electrophoresis, and P genotyping were performed on 10 G2 strains. The antigenic and genetic variation of the gene encoding the major neutralization glycoprotein (VP7) was also investigated by using G2-specific monoclonal antibodies and sequence analysis. Alterations in the characteristic DS-1 genogroup gene constellations were more likely to occur in the VP4 gene, and three genotypes were observed: P[4], P[6], and a dual P[4]-P[6] type. The failure of G2-specific monoclonal antibodies to type African G2 strains was more likely due to improper storage of the original stool, although G2 monotypes were detected. Phylogenetic analyses revealed clusters of serotype G2 strains that were more commonly associated with seasons during which G2 was predominant. No rotavirus vaccine trials have been conducted in an area where G2 strains were the predominant circulating serotype, and the continued surveillance of rotavirus epidemics in Africa will be preparation for future vaccine implementation in an area that clearly needs these preventative medicines.
1999年至2000年期间，在非洲大陆的七个国家分离出了G2血清型轮状病毒株。为了研究相关的DS-1基因组特征，对10株G2病毒株进行了亚组（VP6）酶联免疫吸附测定、聚丙烯酰胺凝胶电泳和P基因分型。还使用G2特异性单克隆抗体和序列分析研究了编码主要中和糖蛋白（VP7）的基因的抗原性和遗传变异。特征性DS-1基因组基因组合的改变更有可能发生在VP4基因中，观察到三种基因型：P[4]、P[6]和双重P[4]-P[6]型。尽管检测到G2单型，但G2特异性单克隆抗体无法对非洲G2病毒株进行分型，这更可能是由于原始粪便储存不当所致。系统发育分析揭示了G2血清型病毒株的聚类，这些聚类更常与G2占主导地位的季节相关。在G2病毒株为主要流行血清型的地区尚未进行轮状病毒疫苗试验，非洲对轮状病毒流行情况的持续监测将为未来在明显需要这些预防药物的地区实施疫苗做好准备。
