Rotavirus Group, National Institute of Virology, Pune, India.
J Med Virol. 2010 Mar;82(3):519-27. doi: 10.1002/jmv.21708.
A total of 1,591 fecal specimens were collected in 1993-1996 and 2004-2007 from adolescents and adults with acute gastroenteritis in Pune, India for detection and characterization of rotavirus. At the two time points, group A rotavirus was detected in 8.6% and 16.2% of the adolescents and 5.2% and 17.2% of the adults, respectively. Reverse transcription-PCR with consensus primers followed by multiplex genotyping PCR detected common strains G1P[8], G2P[4], G3P[8], and G4P[8] in a total of 53.1% of the samples from 1993 to 1996, while the only prevalent strain identified in 2004-2007 was G2P[4] (23.5% of total). Uncommon rotavirus strains (G1P[4], G2P[8] G9P[6]/P[4]) increased from 7.8% (1993-1996) to 41.2% (2004-2007), while the prevalence of mixed rotavirus infections was high (39%/35%) at both time points. Mixed infections detected by multiplex PCR were confirmed by sequencing two or more individual genotype-specific PCR products of the VP7 and VP4 genes from the same sample. Phylogenetic analysis of the sequences showed circulation of a heterogeneous rotavirus strain population comprising genotypes G1 (lineages I and IIb), G2 (lineages I and IIb), G4 (lineage Ia), P[4] (lineages P[4]-5 and P[4]-1), P[8] (lineages P[8]-II and P[8]-III), and P[6] (M37-like lineage). The VP6 gene sequences of the nontypeable strains were most homologous to animal strains. This study documents the molecular epidemiology of rotavirus strains in adolescents and adults in India, and suggests that it may be important to monitor these strains over time for the potential impact on rotavirus vaccines under development for use in the Indian population. J. Med. Virol. 82:519-527, 2010. (c) 2010 Wiley-Liss, Inc.
1993-1996 年和 2004-2007 年，从印度浦那患有急性肠胃炎的青少年和成年人中收集了 1591 份粪便标本，用于检测和鉴定轮状病毒。在这两个时间点，A 组轮状病毒分别在青少年中检测到 8.6%和 16.2%，在成年人中检测到 5.2%和 17.2%。使用通用引物进行逆转录-PCR，然后进行多重基因分型 PCR，共检测到 53.1%的样本中存在常见的 G1P[8]、G2P[4]、G3P[8]和 G4P[8]菌株，而在 2004-2007 年仅鉴定出流行的 G2P[4]（占总样本的 23.5%）。罕见的轮状病毒株（G1P[4]、G2P[8]、G9P[6]/P[4]）从 1993-1996 年的 7.8%增加到 2004-2007 年的 41.2%，而混合感染的发生率很高（39%/35%）。通过多重 PCR 检测到的混合感染通过对同一样本的 VP7 和 VP4 基因的两个或更多个个体基因型特异性 PCR 产物进行测序来确认。序列的系统进化分析显示，存在由基因型 G1（谱系 I 和 IIb）、G2（谱系 I 和 IIb）、G4（谱系 Ia）、P[4]（谱系 P[4]-5 和 P[4]-1）、P[8]（谱系 P[8]-II 和 P[8]-III）和 P[6]（M37 样谱系）组成的异质轮状病毒株群体循环。无法分型株的 VP6 基因序列与动物株最同源。本研究记录了印度青少年和成年人中轮状病毒株的分子流行病学情况，并表明随着时间的推移，监测这些株的情况可能对正在开发用于印度人群的轮状病毒疫苗产生潜在影响非常重要。J. Med. Virol. 82:519-527, 2010.（c）2010 Wiley-Liss, Inc.
