将gus嵌合基因直接导入成熟豇豆胚后其瞬时表达及组织学定位
Transient expression and histological localization of a gus chimeric gene after direct transfer to mature cowpea embryos.
作者信息
Penza R, Akella V, Lurquin P F
机构信息
Department of Genetics and Cell Biology, Washington State University, Pullman 99164-4234.
出版信息
Biotechniques. 1992 Oct;13(4):576, 578, 580.
PMID:1476727
Abstract
Transient expression of a chimeric gus reporter gene was demonstrated in cowpea embryonic cells after direct gene transfer. This report shows that seed-derived embryos express the reporter gene at high frequency after passive or electroporation-mediated DNA transfer.
摘要
直接基因转移后，在豇豆胚胎细胞中证实了嵌合gus报告基因的瞬时表达。本报告表明，在被动或电穿孔介导的DNA转移后，种子来源的胚胎高频表达报告基因。
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