Department of Genetics and Cell Biology, Washington State University, 99164-4234, Pullman, WA, USA.
Plant Cell Rep. 1993 Jan;12(2):110-7. doi: 10.1007/BF00241945.
Cowpea (Vigna unguiculata Walp) embryos mechanically isolated from mature seeds and incubated in the presence of plasmid DNA harboring chimeric gus genes were shown to germinate into seedlings expressing β-glucuronidase activity in a variety of tissues, including the apical meristem. Embryo electroporation in the presence of DNA and protectants such as spermine and Lipofectin(TM) increased both the proportion of embryo-derived seedlings expressing the chimeric gene and the level of gene expression. Microscopic observations of thin sections showed that the blue crystals representing the end product of transgene activity on X-glu were exclusively located inside the treated cells. Histological localization of the blue dye crystals varied with the promoter used to drive the transgene.
从成熟种子中机械分离出豇豆（Vigna unguiculata Walp）胚胎，并在含有嵌合 gus 基因的质粒 DNA 存在的情况下孵育，结果表明，这些胚胎在各种组织中发芽成表达β-葡萄糖醛酸酶活性的幼苗，包括顶端分生组织。在 DNA 和保护剂（如亚精胺和 Lipofectin（TM））存在的情况下对胚胎进行电穿孔，既增加了表达嵌合基因的胚胎衍生幼苗的比例，又提高了基因表达水平。对薄片的显微镜观察表明，代表转基因活性的末端产物 X-glu 的蓝色晶体仅位于处理过的细胞内。蓝色染料晶体的组织学定位随启动子的不同而不同，启动子用于驱动转基因。
