Yu Kang, Jiang Song-Fu, Lin Mao-Fang, Wu Jian-Bo, Lin Jing
Department of Hematology, The First Affiliated Hospital of Wenzhou Medical College, China.
Lab Invest. 2004 Mar;84(3):390-2. doi: 10.1038/labinvest.3700042.
Intestinal trefoil factor (TFF3/ITF), a member of trefoil factor family (TFF) domain peptides, is normally expressed by goblet cells and secreted into the lumen of the intestinal tract, and plays an important role in the maintenance and repair of the intestinal mucosal barrier. Significant amounts of TFF3/ITF are present in the meconium of human infants. Here, we describe a relatively simple method for extraction and purification of natural human TFF3/ITF from meconium. The purification methods include acid treatment, ammonium sulfate precipitation, isoelectric precipitation, and iron exchange chromatography. A relatively large amount of natural TFF3/ITF can be purified from meconium of full-term infants and this purified, natural TFF3/ITF is biologically active in an ethanol-induced rat gastric mucosal injury model.
肠三叶因子（TFF3/ITF）是三叶因子家族（TFF）结构域肽的成员之一，通常由杯状细胞表达并分泌到肠道管腔中，在维持和修复肠道黏膜屏障方面发挥重要作用。人类婴儿的胎粪中存在大量的TFF3/ITF。在此，我们描述了一种从胎粪中提取和纯化天然人TFF3/ITF的相对简单的方法。纯化方法包括酸处理、硫酸铵沉淀、等电沉淀和离子交换色谱法。从足月婴儿的胎粪中可以纯化出相对大量的天然TFF3/ITF，并且这种纯化的天然TFF3/ITF在乙醇诱导的大鼠胃黏膜损伤模型中具有生物活性。
