绿茶儿茶素与硫糖铝联合对蒙古沙鼠幽门螺杆菌感染的抑制作用

Inhibitory effect of green tea catechins in combination with sucralfate on Helicobacter pylori infection in Mongolian gerbils.

作者信息

Takabayashi Fumiyo, Harada Noboru, Yamada Masami, Murohisa Binzaburo, Oguni Itaro

机构信息

Shizuoka College, University of Shizuoka, 2-2-1 Oshika, Shizuoka 422-8021, Japan.

出版信息

J Gastroenterol. 2004 Jan;39(1):61-3. doi: 10.1007/s00535-003-1246-0.

DOI:10.1007/s00535-003-1246-0

PMID:14767736

Abstract

BACKGROUND

The occurrence of antibiotic-resistant Helicobacter pylori has been reported. It is desirable to develop an effective method to prevent the occurrence of resistant strains of Helicobacter pylori. Green tea catechins (GTCs) have been reported to have an antibacterial effect. Therefore, the possibility of eradicating Helicobacter pylori by the oral administration of GTCs was investigated.

METHODS

Solutions of GTCs and solutions of GTCs adsorbed to sucralfate (GTC-scf), at concentrations of 20 mg GTCs and/or 20 mg sucralfate/ml were prepared. Then 1 ml of the GTC-scf or the GTC solution was administered daily, for 10 days to Mongolian gerbils infected with Helicobacter pylori. Then the stomachs were extirpated and homogenized. The homogenate was spread on selective medium plates. After 5-day culture, colony-forming units (CFU) of Helicobacter pylori were counted.

RESULTS

The CFU of Helicobacter pylori was significantly decreased by GTC-scf.

CONCLUSIONS

GTC-scf may have a bactericidal effect on Helicobacter pylori infection.

摘要

背景

已有报道出现了对抗生素耐药的幽门螺杆菌。开发一种有效的方法来预防幽门螺杆菌耐药菌株的出现是很有必要的。据报道，绿茶儿茶素（GTCs）具有抗菌作用。因此，研究了口服GTCs根除幽门螺杆菌的可能性。

方法

制备浓度为20mg GTCs和/或20mg硫糖铝/毫升的GTCs溶液以及吸附有硫糖铝的GTCs溶液（GTC-scf）。然后每天给感染幽门螺杆菌的蒙古沙鼠灌胃1毫升GTC-scf或GTC溶液，持续10天。然后取出胃并匀浆。将匀浆液接种在选择性培养基平板上。培养5天后，计数幽门螺杆菌的菌落形成单位（CFU）。