厚朴酚与克拉霉素联合应用对克拉霉素耐药幽门螺杆菌的体内外作用
Effect of plaunotol in combination with clarithromycin against clarithromycin-resistant Helicobacter pylori in vitro and in vivo.
作者信息
Sasaki Makoto, Mizoshita Tsutomu, Mizushima Takashi, Inoue Harumi, Kamiya Takeshi, Kataoka Hiromi, Ogaswara Naotaka, Wada Tsuneya, Kubota Eiji, Mori Yoshinori, Shimura Takaya, Hirata Hirokazu, Ando Kenji, Okamoto Yasuyuki, Ohara Hirotaka, Nakao Haruhisa, Joh Takashi
机构信息
Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho, Nagoya 467-8601, Japan.
出版信息
J Antimicrob Chemother. 2007 Nov;60(5):1060-3. doi: 10.1093/jac/dkm329. Epub 2007 Sep 7.
OBJECTIVES
Recently, there has been a decrease in the eradication rate of Helicobacter pylori due to the increase in antibiotic resistance of this bacterium. Plaunotol, a cytoprotective anti-ulcer agent, exhibits antibacterial activity against H. pylori. The purpose of the present study was to investigate the effect of plaunotol in combination with clarithromycin against clarithromycin-resistant H. pylori clinical isolates.
METHODS AND RESULTS
In the chequerboard titration method, the combination of plaunotol and clarithromycin showed a synergistic effect against 67% (10/15) clarithromycin-resistant strains and an additive effect against the other strains. No indifferent and antagonistic effects were observed against any of the strains tested. In a gastritis model of Mongolian gerbils infected with clarithromycin-resistant H. pylori, the plaunotol (40 mg/kg) and clarithromycin (66.6 mg/kg) combination exhibited synergistic effects; however, neither plaunotol nor clarithromycin alone showed bactericidal effects.
CONCLUSIONS
These results suggest that plaunotol may play a useful role in combination with anti-H. pylori drugs in the treatment of diseases associated with clarithromycin-resistant H. pylori.
目的
近年来，由于幽门螺杆菌抗生素耐药性增加，其根除率有所下降。普拉诺托尔是一种具有细胞保护作用的抗溃疡药物，对幽门螺杆菌具有抗菌活性。本研究的目的是探讨普拉诺托尔与克拉霉素联合使用对克拉霉素耐药幽门螺杆菌临床分离株的作用。
方法与结果
棋盘滴定法显示，普拉诺托尔与克拉霉素联合使用对67%（10/15）的克拉霉素耐药菌株有协同作用，对其他菌株有相加作用。对所测试的任何菌株均未观察到无关和拮抗作用。在感染克拉霉素耐药幽门螺杆菌的蒙古沙鼠胃炎模型中，普拉诺托尔（40mg/kg）与克拉霉素（66.6mg/kg）联合使用具有协同作用；然而，单独使用普拉诺托尔或克拉霉素均未显示出杀菌作用。
结论
这些结果表明，普拉诺托尔与抗幽门螺杆菌药物联合使用可能在治疗与克拉霉素耐药幽门螺杆菌相关的疾病中发挥有益作用。
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