[Rapid detection of 18-trisomy syndrome using primed in situ labeling technique].
作者信息
Yang Jian-bin, Zheng Shu
机构信息
Cancer Institute, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Feb;21(1):74-6.
OBJECTIVE
To develop a rapid method for the detection of chromosomes and try to verify the feasibility of using modified primed in situ labeling (PRINS) technique for rapid detection of the interphase nuclei of uncultured amniocytes.
METHODS
Chromosome 18 was detected and analyzed by the modified PRINS in 262 amniotic fluid samples.
RESULTS
The specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 95% of the samples, PRINS reactions with primer 18cen were successfully induced. Two samples were properly identified and correctly scored as trisomic 18.
CONCLUSION
The results suggest that PRINS technique is simple, rapid and cost-effective. It is sensitive, specific, and thus can enhance the accuracy of standard cytogenetic analysis.
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