Ni B, Li H, Zou Y, Wu S
Hunan Provincial Key Laboratory of Medical Genetics, Family Planning Institute of Hunan Province, Changsha, Hunan, 410005 P. R. China
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Dec 10;15(6):373-5.
To develop a rapid method for detection of chromosomes.
Chromosomes X, 18 were detected by the primed in situ labeling(PRINS) in eight samples of female peripheral blood cultures.
Specific chromosomes were obtained on both metaphase and interphase nuclei. The efficiency of labeling was 84%-92%(mean=89%) in chromosome 18, and 73%-87%(mean=84%) in chromosome X. The pretreatment of slides by proteinase K markedly raised the efficiency of labeling and increased the intensity of signals. PRINS reaction could be performed automatically with a programmable thermocycler in less than one hour.
The results suggest that PRINS is a fast and specific method for identifying chromosomes. It may be a reliable technique for detecting aneuploid in prenatal diagnosis.
