[Use of the primed in situ labeling technique for a rapid detection of chromosomes X, 18]. - Suppr | 超能文献

[Use of the primed in situ labeling technique for a rapid detection of chromosomes X, 18].

作者信息

Ni B, Li H, Zou Y, Wu S

机构信息

Hunan Provincial Key Laboratory of Medical Genetics, Family Planning Institute of Hunan Province, Changsha, Hunan, 410005 P. R. China

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Dec 10;15(6):373-5.

Abstract

OBJECTIVE

To develop a rapid method for detection of chromosomes.

METHODS

Chromosomes X, 18 were detected by the primed in situ labeling(PRINS) in eight samples of female peripheral blood cultures.

RESULTS

Specific chromosomes were obtained on both metaphase and interphase nuclei. The efficiency of labeling was 84%-92%(mean=89%) in chromosome 18, and 73%-87%(mean=84%) in chromosome X. The pretreatment of slides by proteinase K markedly raised the efficiency of labeling and increased the intensity of signals. PRINS reaction could be performed automatically with a programmable thermocycler in less than one hour.

CONCLUSION

The results suggest that PRINS is a fast and specific method for identifying chromosomes. It may be a reliable technique for detecting aneuploid in prenatal diagnosis.

摘要

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